› 9e8l

Electron Microscopy
3.59Å resolution

Nub1/Fat10-processing human 26S proteasome bound to Txnl1 with Rpt4 at top of spiral staircase

Released:
DOI: 10.2210/pdb9e8l/pdb
PDB entry 9e8l coloured by chain, front view.
PDB entry 9e8l coloured by chain, side view.
PDB entry 9e8l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural landscape of AAA+ ATPase motor states in the substrate-degrading human 26S proteasome reveals conformation-specific binding of TXNL1.
Arkinson C, Gee CL, Zhang Z, Dong KC, Martin A
bioRxiv (2024)
PMID: 39574680
Related structures: EMD-47724

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
PDBe Complex ID:
PDB-CPX-281391 (preferred)
Entry contents:
28 distinct polypeptide molecules
Macromolecules (28 distinct):
26S proteasome regulatory subunit 7 Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 48.7 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P35998 (Residues: 1-433; Coverage: 100%)
Gene names: MSS1, PSMC2
Sequence domains:
26S proteasome regulatory subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 440 amino acids
Theoretical weight: 49.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62191 (Residues: 1-440; Coverage: 100%)
Gene name: PSMC1
Sequence domains:
26S proteasome regulatory subunit 8 Chain: C
26S proteasome regulatory subunit 6B Chain: D
26S proteasome regulatory subunit 10B Chain: E
26S proteasome regulatory subunit 6A Chain: F
Proteasome subunit alpha type-6 Chain: G
Proteasome subunit alpha type-2 Chain: H
Proteasome subunit alpha type-4 Chain: I
Proteasome subunit alpha type-7 Chain: J
Proteasome subunit alpha type-5 Chain: K
Proteasome subunit alpha type-1 Chain: L
Proteasome subunit alpha type-3 Chain: M
26S proteasome non-ATPase regulatory subunit 1 Chain: U
26S proteasome non-ATPase regulatory subunit 3 Chain: V
26S proteasome non-ATPase regulatory subunit 12 Chain: W
26S proteasome non-ATPase regulatory subunit 11 Chain: X
26S proteasome non-ATPase regulatory subunit 6 Chain: Y
26S proteasome non-ATPase regulatory subunit 7 Chain: Z
26S proteasome non-ATPase regulatory subunit 13 Chain: a
26S proteasome non-ATPase regulatory subunit 4 Chain: b
26S proteasome non-ATPase regulatory subunit 14 Chain: c
26S proteasome non-ATPase regulatory subunit 8 Chain: d
26S proteasome complex subunit SEM1 Chain: e
26S proteasome non-ATPase regulatory subunit 2 Chain: f
NEDD8 ultimate buster 1 Chain: g
Thioredoxin-like protein 1 Chain: u
substrate peptide Chain: v

Ligands and Environments

4 bound ligands:
Ligand ADP 4 x ADP
Ligand MG 3 x MG
Ligand ATP 2 x ATP
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.59Å
Relevant EMDB volumes: EMD-47724
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)

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