9eh0

Electron Microscopy
3.6Å resolution

RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome, 30 bp upstream

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
PDBe Complex ID:
PDB-CPX-285138 (preferred)
Entry contents:
28 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (31 distinct):
DNA-directed RNA polymerase subunit Chain: A
Molecule details ›
Chain: A
Length: 1984 amino acids
Theoretical weight: 219.05 KDa
Source organism: Sus scrofa
DNA-directed RNA polymerase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 1251 amino acids
Theoretical weight: 142.43 KDa
Source organism: Sus scrofa
DNA-directed RNA polymerase II subunit RPB3 Chain: C
RNA polymerase Rpb4/RPC9 core domain-containing protein Chain: D
DNA-directed RNA polymerase II subunit E Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerase II subunit RPB7 Chain: G
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
RNA polymerase II subunit J Chain: K
RNA polymerase II subunit K Chain: L
Transcription elongation factor SPT6 Chain: M
Protein IWS1 homolog Chain: O
RNA polymerase-associated protein CTR9 homolog Chain: Q
RNA polymerase-associated protein RTF1 homolog Chain: R
Transcription elongation factor A protein 1 Chain: S
RNA polymerase-associated protein LEO1 Chain: U
RNA polymerase II-associated factor 1 homolog Chain: V
WDR61 Chain: W
Parafibromin Chain: X
Transcription elongation factor SPT4 Chain: Y
Transcription elongation factor SPT5 Chain: Z
Histone H3 Chains: a, e
Histone H4 Chains: b, f
Histone H2A type 1 Chains: c, g
Histone H2B 1.1 Chains: d, h
Histone-lysine N-methyltransferase SETD2 Chain: l
Non-template Chain: N
Template DNA Chain: T
RNA Chain: P

Ligands and Environments

2 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-48042
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided