› 9enb

Electron Microscopy
2.66Å resolution

Human pseudouridine synthase 3 (PUS3 R116A mutant) and two tRNA-Gln

Released:
DOI: 10.2210/pdb9enb/pdb
PDB entry 9enb coloured by chain, front view.
PDB entry 9enb coloured by chain, side view.
PDB entry 9enb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The molecular basis of tRNA selectivity by human pseudouridine synthase 3.
Lin TY, Kleemann L, Jeżowski J, Dobosz D, Rawski M, Indyka P, Ważny G, Mehta R, Chramiec-Głąbik A, Koziej Ł, Ranff T, Fufezan C, Wawro M, Kochan J, Bereta J, Leidel SA, Glatt S
Mol Cell 84 2472-2489.e8 (2024)
PMID: 38996458
Related structures: EMD-19830

Function and Biology Details

Reaction catalysed: 
tRNA uridine(38/39) = tRNA pseudouridine(38/39)
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-250383 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA pseudouridine(38/39) synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 481 amino acids
Theoretical weight: 55.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BZE2 (Residues: 1-481; Coverage: 100%)
Gene names: FKSG32, PUS3
Sequence domains: tRNA pseudouridine synthase
tRNA-Gln Chains: C, D
Molecule details ›
Chains: C, D
Length: 75 nucleotides
Theoretical weight: 24.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.66Å
Relevant EMDB volumes: EMD-19830
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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