› 9eoy

X-ray diffraction
2.1Å resolution

Structure of Thr354Asn, Glu355Gln, Thr412Asn, Ile414Met, Ile464His, and Phe467Met mutant human CaMKII alpha hub bound to PIPA

Released:
DOI: 10.2210/pdb9eoy/pdb
PDB entry 9eoy coloured by chain, front view.
PDB entry 9eoy coloured by chain, side view.
PDB entry 9eoy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ligand-induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity.
Narayanan D, Larsen ASG, Gauger SJ, Adafia R, Hammershøi RB, Hamborg L, Bruus-Jensen J, Griem-Krey N, Gee CL, Frølund B, Stratton MM, Kuriyan J, Kastrup JS, Langkilde AE, Wellendorph P, Solbak SMØ
Protein Sci 33 e5152 (2024)
PMID: 39275999

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium/calmodulin-dependent protein kinase type II subunit alpha Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 135 amino acids
Theoretical weight: 15.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UQM7 (Residues: 345-475; Coverage: 27%)
Gene names: CAMK2A, CAMKA, KIAA0968
Sequence domains: Calcium/calmodulin dependent protein kinase II association domain

Ligands and Environments

3 bound ligands:
Ligand ACT 6 x ACT
Ligand A1H6K 2 x A1H6K
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2221
Unit cell:
a: 103.045Å b: 182.918Å c: 107.756Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.251
Expression system: Escherichia coli BL21(DE3)

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