› 9euo

Electron Microscopy
3.2Å resolution

Outward-open structure of Drosophila dopamine transporter bound to an atypical non-competitive inhibitor

Released:
DOI: 10.2210/pdb9euo/pdb
PDB entry 9euo coloured by chain, front view.
PDB entry 9euo coloured by chain, side view.
PDB entry 9euo coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of the dopamine transporter with a novel atypical non-competitive inhibitor bound to the orthosteric site.
Pedersen CN, Yang F, Ita S, Xu Y, Akunuri R, Trampari S, Neumann CMT, Desdorf LM, Schiøtt B, Salvino JM, Mortensen OV, Nissen P, Shahsavar A
J Neurochem (2024)
PMID: 39010681
Related structures: EMD-19978

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-247468 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium-dependent dopamine transporter Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 60.94 KDa
Source organism: Drosophila melanogaster
Expression system: Homo sapiens
9D5 ANTIBODY, LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 237 amino acids
Theoretical weight: 25.84 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
9D5 ANTIBODY, HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 240 amino acids
Theoretical weight: 25.92 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

6 bound ligands:
Ligand A1H8F 1 x A1H8F
Ligand Y01 1 x Y01
Ligand 144 1 x 144
Ligand CLR 1 x CLR
Ligand NA 1 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-19978
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

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