› 9evp

Electron Microscopy
3.12Å resolution

SV40 LTAg assembly with DNA in presence of AMPPNP and Mg2+.

Released:
DOI: 10.2210/pdb9evp/pdb
PDB entry 9evp coloured by chain, front view.
PDB entry 9evp coloured by chain, side view.
PDB entry 9evp coloured by chain, top view.
Source organisms:
Primary publication:
Structural dynamics of DNA unwinding by a replicative helicase.
Shahid T, Danazumi AU, Tehseen M, Alhudhali L, Clark AR, Savva CG, Hamdan SM, De Biasio A
Nature (2025)
PMID: 40108462
Related structures: EMD-50010

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-291561 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Large T antigen Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 362 amino acids
Theoretical weight: 41.81 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P03070 (Residues: 266-627; Coverage: 51%)
Sequence domains: Polyomavirus large T antigen C-terminus
DNA Chain: S
Molecule details ›
Chain: S
Length: 7 nucleotides
Theoretical weight: 2.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ANP 6 x ANP
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.12Å
Relevant EMDB volumes: EMD-50010
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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