› 9f45

Electron Microscopy
3.74Å resolution

cryo-EM structure of human LST2 bound to human mTOR complex 1

Released:
DOI: 10.2210/pdb9f45/pdb
PDB entry 9f45 coloured by chain, front view.
PDB entry 9f45 coloured by chain, side view.
PDB entry 9f45 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
mTORC1 phosphorylates and stabilizes LST2 to negatively regulate EGFR.
Battaglioni S, Craigie LM, Filippini S, Maier T, Hall MN
Proc Natl Acad Sci U S A 121 e2405959121 (2024)
PMID: 39141345
Related structures: EMD-50184

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273741 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Serine/threonine-protein kinase mTOR Chains: A, B
Molecule details ›
Chains: A, B
Length: 2549 amino acids
Theoretical weight: 289.26 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42345 (Residues: 1-2549; Coverage: 100%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains:
Target of rapamycin complex subunit LST8 Chains: C, D
Molecule details ›
Chains: C, D
Length: 326 amino acids
Theoretical weight: 35.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BVC4 (Residues: 1-326; Coverage: 100%)
Gene names: GBL, LST8, MLST8
Sequence domains: WD domain, G-beta repeat
Regulatory-associated protein of mTOR Chains: E, F
Molecule details ›
Chains: E, F
Length: 1363 amino acids
Theoretical weight: 152.76 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8N122 (Residues: 1-1335; Coverage: 100%)
Gene names: KIAA1303, RAPTOR, RPTOR
Sequence domains:
Lateral signaling target protein 2 homolog Chains: G, H
Molecule details ›
Chains: G, H
Length: 887 amino acids
Theoretical weight: 96.6 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9HCC9 (Residues: 1-887; Coverage: 100%)
Gene names: KIAA1643, LST2, ZFYVE28
Sequence domains: FYVE zinc finger

Ligands and Environments

1 bound ligand:
Ligand IHP 2 x IHP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.74Å
Relevant EMDB volumes: EMD-50184
Expression system: Spodoptera frugiperda

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