› 9f5z

Electron Microscopy
2.39Å resolution

Structure of the Chlamydomonas reinhardtii respiratory complex III from respiratory supercomplex

Released:
DOI: 10.2210/pdb9f5z/pdb
PDB entry 9f5z coloured by chain, front view.
PDB entry 9f5z coloured by chain, side view.
PDB entry 9f5z coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
In-cell architecture of the mitochondrial respiratory chain.
Waltz F, Righetto RD, Lamm L, Salinas-Giegé T, Kelley R, Zhang X, Obr M, Khavnekar S, Kotecha A, Engel BD
Science 387 1296-1301 (2025)
PMID: 40112058
Related structures: EMD-50204

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-289200 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Cytochrome b Chains: 1A, 1B
Molecule details ›
Chains: 1A, 1B
Length: 381 amino acids
Theoretical weight: 42.36 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P23662 (Residues: 1-381; Coverage: 100%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: 1C, 1D
Molecule details ›
Chains: 1C, 1D
Length: 262 amino acids
Theoretical weight: 28.55 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q8HEB4 (Residues: 1-262; Coverage: 100%)
Gene names: CHLRE_01g051900v5, risp
Sequence domains:
Cytochrome c domain-containing protein Chains: 1E, 1F
Complex III subunit 9 Chains: 1G, 1H
Cytochrome b-c1 complex subunit 6 Chains: 1I, 1J
Mitochondrial ubiquinol-cytochrome c oxidoreductase subunit 8 Chains: 1K, 1L
MPP-Beta Chains: 1M, 1N
Mitochondrial ubiquinol-cytochrome c oxidoreductase subunit 10 Chains: 1O, 1P
Alpha-MPP Chains: 1Q, 1S
Cytochrome b-c1 complex subunit 7 Chains: 1R, 1T

Ligands and Environments


Cofactor: Ligand HEC 6 x HEC

Cofactor: Ligand UQ5 4 x UQ5
5 bound ligands:
Ligand CDL 8 x CDL
Ligand 3PH 7 x 3PH
Ligand PTY 2 x PTY
Ligand PC7 2 x PC7
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.39Å
Relevant EMDB volumes: EMD-50204

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