› 9f8x

X-ray diffraction
1.5Å resolution

Low-dose structure of Marinobacter nauticus nitrous oxide reductase

Released:
DOI: 10.2210/pdb9f8x/pdb
PDB entry 9f8x coloured by chain, front view.
PDB entry 9f8x coloured by chain, side view.
PDB entry 9f8x coloured by chain, top view.
Source organism: Marinobacter nauticus
Primary publication:
Revisiting the metal sites of nitrous oxide reductase in a low-dose structure from Marinobacter nauticus.
Pomowski A, Dell'Acqua S, Wüst A, Pauleta SR, Moura I, Einsle O
J Biol Inorg Chem 29 279-290 (2024)
PMID: 38720157

Function and Biology Details

Reaction catalysed: 
Nitrogen + H(2)O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-247256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrous-oxide reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 631 amino acids
Theoretical weight: 70.46 KDa
Source organism: Marinobacter nauticus
UniProt:
  • Canonical: A0A368XMX8 (Residues: 1-631; Coverage: 100%)
Gene names: DET61_107128, nosZ
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand CUA 4 x CUA
Ligand CUK 4 x CUK
Ligand CA 8 x CA
Ligand CL 4 x CL
Ligand EDO 13 x EDO
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 65.26Å b: 69.375Å c: 153.188Å
α: 81.384° β: 77.918° γ: 88.608°
R-values:
R R work R free
0.131 0.13 0.152

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