› 9f93

X-ray diffraction
1.54Å resolution

Complex of phenazine biosynthesis enzyme PhzF with 2-amino-5-(4-fluorophenyl)benzoic acid

Released:
DOI: 10.2210/pdb9f93/pdb
PDB entry 9f93 coloured by chain, front view.
PDB entry 9f93 coloured by chain, side view.
PDB entry 9f93 coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Substrate-Based Ligand Design for Phenazine Biosynthesis Enzyme PhzF.
Baumgarten J, Schneider P, Thiemann M, Zimmermann M, Diederich C, Blankenfeldt W, Kunick C
ChemMedChem e202400466 (2024)
PMID: 39163032

Function and Biology Details

Reaction catalysed: 
(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxyate = (1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-283593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trans-2,3-dihydro-3-hydroxyanthranilate isomerase Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 32.26 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O69754 (Residues: 1-278; Coverage: 100%)
Gene names: PA1904, PA4215, phzF, phzF1, phzF2
Sequence domains: Phenazine biosynthesis-like protein

Ligands and Environments

2 bound ligands:
Ligand A1IAV 1 x A1IAV
Ligand BU3 1 x BU3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P3221
Unit cell:
a: 56.059Å b: 56.059Å c: 155.163Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.162 0.186
Expression system: Escherichia coli BL21(DE3)

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