› 9f9f

X-ray diffraction
1.8Å resolution

Laser excitation effects on BR: Extrapolated 6 ps Light dataset recorded at 2493 GW/cm2 at SwissFEL

Released:
DOI: 10.2210/pdb9f9f/pdb
Model geometry
Fit model/data
PDB entry 9f9f coloured by chain, front view.
PDB entry 9f9f coloured by chain, side view.
PDB entry 9f9f coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate.
Bertrand Q, Nogly P, Nango E, Kekilli D, Khusainov G, Furrer A, James D, Dworkowski F, Skopintsev P, Mous S, Martiel I, Börjesson P, Ortolani G, Huang CY, Kepa M, Ozerov D, Brünle S, Panneels V, Tanaka T, Tanaka R, Tono K, Owada S, Johnson PJM, Nass K, Knopp G, Cirelli C, Milne C, Schertler G, Iwata S, Neutze R, Weinert T, Standfuss J
Nat Commun 15 10278 (2024)
PMID: 39604356

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 25.26 KDa
Source organism: Halobacterium salinarum
Expression system: Escherichia coli
UniProt:
  • Canonical: P02945 (Residues: 18-247; Coverage: 88%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein

Ligands and Environments

3 bound ligands:
Ligand LI1 11 x LI1
Ligand OLC 4 x OLC
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SwissFEL ARAMIS BEAMLINE ESA
Spacegroup: P63
Unit cell:
a: 62.95Å b: 62.95Å c: 111.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.216 0.259
Expression system: Escherichia coli

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