› 9fii

X-ray diffraction
2.5Å resolution

Crystal Structure of oxidized NuoEF variant E222K(NuoF) from Aquifex aeolicus

Released:
DOI: 10.2210/pdb9fii/pdb
PDB entry 9fii coloured by chain, front view.
PDB entry 9fii coloured by chain, side view.
PDB entry 9fii coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structural robustness of the NADH binding site in NADH:ubiquinone oxidoreductase (complex I).
Göppert-Asadollahpour S, Wohlwend D, Friedrich T
Biochim Biophys Acta Bioenerg 1865 149491 (2024)
PMID: 38960077

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130389 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NADH-quinone oxidoreductase subunit E Chains: A, C
Molecule details ›
Chains: A, C
Length: 160 amino acids
Theoretical weight: 18.57 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O66842 (Residues: 1-160; Coverage: 100%)
Gene names: aq_574, nuoE
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-quinone oxidoreductase subunit F Chains: B, D
Molecule details ›
Chains: B, D
Length: 434 amino acids
Theoretical weight: 48.52 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O66841 (Residues: 1-426; Coverage: 100%)
Gene names: aq_573, nuoF
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand FES 2 x FES
Ligand NA 9 x NA
Ligand SF4 2 x SF4
Ligand FMN 2 x FMN
Ligand GOL 4 x GOL
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 64.072Å b: 113.397Å c: 189.555Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.249
Expression system: Escherichia coli

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