› 9fj3

X-ray diffraction
1.4Å resolution

Structure of ubiquitin bound of coiled-coil UIM form 2

Released:
DOI: 10.2210/pdb9fj3/pdb
PDB entry 9fj3 coloured by chain, front view.
PDB entry 9fj3 coloured by chain, side view.
PDB entry 9fj3 coloured by chain, top view.
Source organisms:
Primary publication:
An engineered ubiquitin binding coiled coil peptide
Vosbein P, Vergara PP, Huang DT, Thomson AR
Chem Sci (2024)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-251514 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin Chains: A, D
Molecule details ›
Chains: A, D
Length: 81 amino acids
Theoretical weight: 8.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
GLU-GLN-GLU-ILE-GLU-GLU-LEU-GLU-ILE-GLU-ILE-ALA-ILE-LEU-LEU-SER-GLU-ILE-GLU-GLY Chains: B, E
Molecule details ›
Chains: B, E
Length: 20 amino acids
Theoretical weight: 2.3 KDa
Source organism: synthetic construct
Expression system: Not provided
LYS-GLN-LYS-ILE-ALA-ALA-LEU-LYS-TYR-LYS-ILE-ALA-ALA-LEU-LYS-GLN-LYS-ILE Chains: C, F
Molecule details ›
Chains: C, F
Length: 20 amino acids
Theoretical weight: 2.25 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand A1IDH 2 x A1IDH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 42.45Å b: 50.09Å c: 52.2Å
α: 90° β: 101.26° γ: 90°
R-values:
R R work R free
0.218 0.217 0.238
Expression systems:
  • Escherichia coli
  • Not provided

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