9frl

Electron Microscopy
2.97Å resolution

Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to SD mRNA with h44 in up position

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 34-mer (preferred)
PDBe Complex ID:
PDB-CPX-283771 (preferred)
Entry contents:
31 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (34 distinct):
Small ribosomal subunit protein eS1 Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.58 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q9UXD4 (Residues: 1-208; Coverage: 100%)
Gene names: SSO0746, rps3ae
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 231 amino acids
Theoretical weight: 26.04 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: P95993 (Residues: 1-231; Coverage: 100%)
Gene names: C05004, C05_043, SSO0067, rps2, rps2Ab
Sequence domains: Ribosomal protein S2
Small zinc finger protein HVO-2753-like zinc-binding pocket domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 65 amino acids
Theoretical weight: 7.35 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q980V0 (Residues: 1-65; Coverage: 100%)
Gene name: SSO5343
Sequence domains: Small zinc finger protein HVO_2753-like, Zn-binding pocket
Small ribosomal subunit protein uS4 Chain: D
Molecule details ›
Chain: D
Length: 181 amino acids
Theoretical weight: 20.78 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: P95987 (Residues: 1-181; Coverage: 100%)
Gene names: C04_049, SSO0073, rps4, rps4Ab
Sequence domains:
Small ribosomal subunit protein uS5 Chain: F
Molecule details ›
Chain: F
Length: 214 amino acids
Theoretical weight: 23.72 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q9UX87 (Residues: 1-214; Coverage: 100%)
Gene names: C10_032, SSO0698, rps5, rps5Ab
Sequence domains:
Small ribosomal subunit protein eS6 Chain: G
Molecule details ›
Chain: G
Length: 214 amino acids
Theoretical weight: 23.76 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q980A6 (Residues: 1-214; Coverage: 100%)
Gene names: SSO0411, rps6e
Sequence domains: Ribosomal protein S6e
Small ribosomal subunit protein uS7 Chain: H
Molecule details ›
Chain: H
Length: 193 amino acids
Theoretical weight: 22.04 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: P35026 (Residues: 1-193; Coverage: 100%)
Gene names: SSO0217, rps7, rps7Ab
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: I
Molecule details ›
Chain: I
Length: 133 amino acids
Theoretical weight: 15.08 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q9UX92 (Residues: 1-133; Coverage: 100%)
Gene names: C10_027, SSO0703, rps8, rps8Ab
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein eS8 Chain: J
Molecule details ›
Chain: J
Length: 133 amino acids
Theoretical weight: 14.69 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q980W3 (Residues: 1-133; Coverage: 100%)
Gene names: SSO0164, rps8e
Sequence domains: Ribosomal protein S8e
Small ribosomal subunit protein uS9 Chain: K
Molecule details ›
Chain: K
Length: 137 amino acids
Theoretical weight: 15.73 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: P95992 (Residues: 1-137; Coverage: 100%)
Gene names: C05003, SSO0068, rps9, rps9Ab
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: L
Molecule details ›
Chain: L
Length: 102 amino acids
Theoretical weight: 12.13 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: P35027 (Residues: 1-102; Coverage: 100%)
Gene names: SSO0215, rps10, rps10Ab
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: M
Molecule details ›
Chain: M
Length: 132 amino acids
Theoretical weight: 14.07 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: P95988 (Residues: 1-132; Coverage: 100%)
Gene names: C04_050, SSO0072, rps11, rps11Ab
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: N
Molecule details ›
Chain: N
Length: 147 amino acids
Theoretical weight: 16.38 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: P39573 (Residues: 1-147; Coverage: 100%)
Gene names: SSO0219, rps12, rps12Ab
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS13 Chain: O
Molecule details ›
Chain: O
Length: 165 amino acids
Theoretical weight: 18.66 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: P95986 (Residues: 1-165; Coverage: 100%)
Gene names: C04_048, SSO0074, rps13, rps13Ab
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein uS15 Chain: Q
Molecule details ›
Chain: Q
Length: 152 amino acids
Theoretical weight: 17.48 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q980A8 (Residues: 1-152; Coverage: 100%)
Gene names: SSO0408, rps13e, rps15
Sequence domains:
Small ribosomal subunit protein eS17 Chain: S
Molecule details ›
Chain: S
Length: 79 amino acids
Theoretical weight: 9.65 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q980K7 (Residues: 1-79; Coverage: 100%)
Gene names: SSO5561, rps17e
Sequence domains: Ribosomal S17
Small ribosomal subunit protein uS19 Chain: T
Molecule details ›
Chain: T
Length: 140 amino acids
Theoretical weight: 16.4 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q9UXA3 (Residues: 1-140; Coverage: 100%)
Gene names: C10_016, SSO0715, rps19, rps19Ab
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein eS19 Chain: U
Molecule details ›
Chain: U
Length: 158 amino acids
Theoretical weight: 18.15 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q980F7 (Residues: 1-158; Coverage: 100%)
Gene names: SSO0353, rps19E, rps19e
Sequence domains: Ribosomal protein S19e
Small ribosomal subunit protein eS24 Chain: V
Molecule details ›
Chain: V
Length: 120 amino acids
Theoretical weight: 13.12 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q97ZY6 (Residues: 1-120; Coverage: 100%)
Gene names: SSO0435, rps24e
Sequence domains: Ribosomal protein S24e
Small ribosomal subunit protein eS27 Chain: W
Molecule details ›
Chain: W
Length: 66 amino acids
Theoretical weight: 7.4 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q97Z80 (Residues: 1-66; Coverage: 100%)
Gene names: SSO7114, rps27e
Sequence domains: Ribosomal protein S27
Small ribosomal subunit protein eS28 Chain: X
Molecule details ›
Chain: X
Length: 83 amino acids
Theoretical weight: 9.39 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q980Q5 (Residues: 1-83; Coverage: 100%)
Gene names: SSO5479, rps28e
Sequence domains: Ribosomal protein S28e
Small ribosomal subunit protein eS31 Chain: Y
Molecule details ›
Chain: Y
Length: 75 amino acids
Theoretical weight: 8.7 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q97ZY7 (Residues: 1-75; Coverage: 100%)
Gene names: SSO5844, rps27ae
Sequence domains: Ribosomal protein S27a
Large ribosomal subunit protein eL8 Chain: 3
Molecule details ›
Chain: 3
Length: 127 amino acids
Theoretical weight: 13.76 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: P55858 (Residues: 1-127; Coverage: 100%)
Gene names: C04_031, SSO0091, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
aS34 Chain: a
Molecule details ›
Chain: a
Length: 72 amino acids
Theoretical weight: 8.21 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q97ZK4 (Residues: 1-72; Coverage: 100%)
Gene name: SSO6759
LSU ribosomal protein S30E (Rps30E) Chain: e
Molecule details ›
Chain: e
Length: 52 amino acids
Theoretical weight: 6.06 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q97ZH4 (Residues: 1-52; Coverage: 100%)
Gene names: SSO6817, rps30E
Sequence domains: Ribosomal protein S30
Small ribosomal subunit protein uS14 Chain: P
Molecule details ›
Chain: P
Length: 54 amino acids
Theoretical weight: 6.42 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q97ZQ5 (Residues: 1-54; Coverage: 100%)
Gene names: SSO6391, rps14, rps14Ab
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS17 Chain: R
Molecule details ›
Chain: R
Length: 114 amino acids
Theoretical weight: 12.99 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q9UX98 (Residues: 1-114; Coverage: 100%)
Gene names: C10_021, SSO0709, rps17, rps17Ab
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein uS3 Chain: Z
Molecule details ›
Chain: Z
Length: 229 amino acids
Theoretical weight: 25.86 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q9UXA0 (Residues: 1-229; Coverage: 100%)
Gene names: C10_019, SSO0712, rps3, rps3Ab
Sequence domains:
aS33 Chain: d
Molecule details ›
Chain: d
Length: 72 amino acids
Theoretical weight: 8.39 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: D0KTI0 (Residues: 1-72; Coverage: 100%)
Gene name: Ssol_1835
Small ribosomal subunit protein eS25 Chain: c
Molecule details ›
Chain: c
Length: 110 amino acids
Theoretical weight: 12.37 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q97ZZ6 (Residues: 1-110; Coverage: 100%)
Gene names: SSO0425, rps25e
Sequence domains: S25 ribosomal protein
Small ribosomal subunit protein eS4 Chain: E
Molecule details ›
Chain: E
Length: 239 amino acids
Theoretical weight: 27.56 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q9UX94 (Residues: 1-239; Coverage: 100%)
Gene names: C10_025, SSO0705, rps4e
Sequence domains:
rRNA 16S Chain: 2
Molecule details ›
Chain: 2
Length: 1497 nucleotides
Theoretical weight: 486.42 KDa
tRNA Met initiator Chain: 4
Molecule details ›
Chain: 4
Length: 77 nucleotides
Theoretical weight: 24.83 KDa
Messenger RNA Chain: 5
Molecule details ›
Chain: 5
Length: 28 nucleotides
Theoretical weight: 9.03 KDa

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.97Å
Relevant EMDB volumes: EMD-50717