› 9fve

X-ray diffraction
2.81Å resolution

Crystal structure of VcSiaP W73A mutant in complex with sialic acid and a VHH antibody (VHH_VcP#2)

Released:
DOI: 10.2210/pdb9fve/pdb
PDB entry 9fve coloured by chain, front view.
PDB entry 9fve coloured by chain, side view.
PDB entry 9fve coloured by chain, top view.
Source organism: Vicugna pacos
Entry authors: Schneberger N, Hagelueken G

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-575798 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sialic acid-binding periplasmic protein SiaP Chains: A, C, E, G, I, K, M, O, Q, U, W, Y
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q, U, W, Y
Length: 303 amino acids
Theoretical weight: 33.99 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KR64 (Residues: 22-321; Coverage: 100%)
Gene names: VC_1779, siaP
Sequence domains: Bacterial extracellular solute-binding protein, family 7
VHH_VcP#2 Chains: B, D, F, H, J, L, N, P, R, T, V, X
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R, T, V, X
Length: 123 amino acids
Theoretical weight: 13.48 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand SLB 12 x SLB
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: C2
Unit cell:
a: 223.57Å b: 153.113Å c: 210.487Å
α: 90° β: 89.999° γ: 90°
R-values:
R R work R free
0.234 0.234 0.267
Expression system: Escherichia coli

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