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The barley MLA13-AVRA13 heterodimer

Electron Microscopy
3.8Å resolution
PDB entry 9fyc coloured by chain, front view.
PDB entry 9fyc coloured by chain, side view.
PDB entry 9fyc coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CSEP0372 putative effector protein and CC-NBS-LRR resistance protein MLA13
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Multimeric state: hetero dimer
Accessible surface area: 50061.61 Å2
Buried surface area: 2132.61 Å2
Dissociation area: 1,066.3 Å2
Dissociation energy (ΔGdiss): -6.96 kcal/mol
Dissociation entropy (TΔSdiss): 12.2 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-285149

Macromolecules

CC-NBS-LRR resistance protein MLA13
Chain: A
Length: 959 amino acids
Theoretical weight: 108.1 KDa
Source organism: Hordeum vulgare
Expression system: Nicotiana benthamiana
UniProt:
  • Canonical: Q8GSK4 (Residues: 1-959; Coverage: 100%)
Pfam:
CC-NBS-LRR resistance protein MLA13 in PDB entry 9fyc, assembly 1, front view.
CC-NBS-LRR resistance protein MLA13 in PDB entry 9fyc, assembly 1, side view.
CC-NBS-LRR resistance protein MLA13 in PDB entry 9fyc, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q8GSK4  
1959100200300400500600700800900
 
500MDIVTGAISNLIPKLGELLTEEFKLHKGVKKNIEDLGKELESMNAALIKIGEVPREQLDSQDKLWADEVRELSYVIEDVVDKFLVQVDGIKSDDNNNEFEGLMKRTTELLKKVKHKHGIAHAIKDIQEQLQKVADRRDRNKVFVPHPTRTIAIDPCLRALYAEATELVGIYGKRDQGLMRLLSMEGDDASNKRLKKVSIVGFGGLGKTTLARAVYEKIKGDFDCRAFVPVGQNPDMKKVLRDILIDLGNPHSDLAMLDANQLIKKLHEFLENKRYLVIIDDIWDEKLWEGINFAFSNRNNLGSRLITTTRIVSVSNSCCSSDGDSVYQMEPLSVDDSRMLFYKRIFPDENACINEFEQVSRDILKKCGGVPLAIITIASALAGDQKMKPKCEWDILLRSLGSGLTEDNSLEEMRRILSFSYSNLPSNLKTCLLYLCVYPEDSMISRDKLIWKWVAEGFVHHENQGNSLYLLGLNYFNQLINRSMIQPIYNYSGEAYACRVHDMVLDLICNLSNEAKFVNLLDGTGNSMSSQSNCRRLSLQKRNEDHQARPFTDIKSMSRVRSITIFPSAIEVMPSLSRFDVLRVLDLSRCNLGENSSMQLNLKGVGHLTHLRYLGLEGTNISKLPAEIGKLQFLEVLDLENNHNLKELPSTVCNFRRLIYLNLVGCQVVPPVGVLQNLTSIEVLSGILVSLNIIAQELGNLKRLRELNILFNDGSLDLYEGFVKSLCNLHHIESLIIGCNSRETSSFELMDLLGERWVPPVHFREFVSSMPSQLSALRGWIKRDPSHLSNLSELILTSVKEVQQDDVVIIGALSSLRRLCIKSTYQTQRLLVIPADGFRCIVGFHLDCGSATQILFEPGALPRAESVVISLGVRVAKEDGNRGFDLGLQGNLLSLRRDVFVSIYCGGARVGEAKEAEAAVRRALDAHPSHPPIYFEMRPHIAKGAHDDDLCEERRRTDF
UniProt
Q8GSK4
Chains
Secondary structure
Interaction interfaces
Sequence conservation
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CSEP0372 putative effector protein
Chain: B
Length: 122 amino acids
Theoretical weight: 13.73 KDa
Source organism: Blumeria graminis
Expression system: Nicotiana benthamiana
UniProt:
  • Canonical: N1JFM8 (Residues: 1-122; Coverage: 100%)
Gene name: BGHDH14_bghG002861000001001
CSEP0372 putative effector protein in PDB entry 9fyc, assembly 1, front view.
CSEP0372 putative effector protein in PDB entry 9fyc, assembly 1, side view.
CSEP0372 putative effector protein in PDB entry 9fyc, assembly 1, top view.
PDBe-KB: UniProt Coverage View: N1JFM8  
1122102030405060708090100110120
 
50100MKTFQFASIVAGLSFLKTTIAAGDGYITLGMGSIHKNDIYRVAEHMWTIDAYSVPSNNHGSYPIFGEEINGSVTRIFPIVYNGDDWRSGDFYYSVESTEDLSYIKLRYNGARYETCMVSSPE
UniProt
N1JFM8
Chains
Secondary structure
Interaction interfaces
Sequence conservation
Molecule details ›

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