› 9g2a

X-ray diffraction
2.05Å resolution

Staphylococcus aureus MazF in complex with nanobody 4

Released:
DOI: 10.2210/pdb9g2a/pdb
PDB entry 9g2a coloured by chain, front view.
PDB entry 9g2a coloured by chain, side view.
PDB entry 9g2a coloured by chain, top view.
Source organisms:
Entry authors: Prolic-Kalinsek M, Zorzini V, Haesaerts S, Loris R

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274970 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endoribonuclease MazF Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 14.95 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7A4G9 (Residues: 2-120; Coverage: 99%)
Gene names: SA1873, mazF
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
Nanobody 4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 134 amino acids
Theoretical weight: 14.54 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand EPE 2 x EPE
Ligand MPO 2 x MPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P1
Unit cell:
a: 37.97Å b: 51.65Å c: 70.34Å
α: 81.26° β: 81.61° γ: 71.65°
R-values:
R R work R free
0.243 0.24 0.307
Expression system: Escherichia coli BL21(DE3)

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