› 9g5l

X-ray diffraction
1.82Å resolution

Stapylococcus aureus MazF in complex with nanobody 10

Released:
DOI: 10.2210/pdb9g5l/pdb
PDB entry 9g5l coloured by chain, front view.
PDB entry 9g5l coloured by chain, side view.
PDB entry 9g5l coloured by chain, top view.
Source organisms:
Entry authors: Prolic-Kalinsek M, Haesaerts S, Loris R

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-280186 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endoribonuclease MazF Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.95 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2FWI8 (Residues: 2-120; Coverage: 99%)
Gene names: SAOUHSC_02303, mazF
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
Nanobody 10 Chain: B
Molecule details ›
Chain: B
Length: 141 amino acids
Theoretical weight: 15.44 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P41212
Unit cell:
a: 99.04Å b: 99.04Å c: 70.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.228
Expression system: Escherichia coli BL21(DE3)

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