Sample information
Author description:
CryoEM structure of influenza A RNP-like particle double-stranded assembled with a 12-mer RNA.
Source organisms:
Expression system:
Escherichia coli BL21(DE3)
Validation information
Experimental information
Specimen preparation
| Buffer name | pH | Details |
|---|---|---|
| - | 7.5 | 20 mM HEPES 150 mM NaCl 5 mM beta-mercaptoethanol 2 mM methyl-PEG8-NHS |
Vitrification
| Cryogen | Temperature | Instrument | Humidity | Details |
|---|---|---|---|---|
| - | - | FEI VITROBOT MARK IV | 98 | 5 seconds blot at force 0 |
Refinement statistics
Structure solution method:
Electron Microscopy
Resolution:
4 Å
