› 9gbw

Electron Microscopy
3.5Å resolution

Overall structure of the substrate-bound U11 snRNP

Released:
DOI: 10.2210/pdb9gbw/pdb
PDB entry 9gbw coloured by chain, front view.
PDB entry 9gbw coloured by chain, side view.
PDB entry 9gbw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of 5' splice site recognition by the minor spliceosome.
Zhao J, Peter D, Brandina I, Liu X, Galej WP
Mol Cell (2025)
PMID: 39809272
Related structures: EMD-51223

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-286563 (preferred)
Entry contents:
12 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (14 distinct):
U11/U12 small nuclear ribonucleoprotein 25 kDa protein Chain: B
Molecule details ›
Chain: B
Length: 132 amino acids
Theoretical weight: 15.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BV90 (Residues: 1-132; Coverage: 100%)
Gene names: C16orf33, SNRNP25
Sequence domains: Ubiquitin-like domain
U11/U12 small nuclear ribonucleoprotein 35 kDa protein Chain: C
Molecule details ›
Chain: C
Length: 246 amino acids
Theoretical weight: 29.51 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q16560 (Residues: 1-246; Coverage: 100%)
Gene names: HM1, SNRNP35, U1SNRNPBP
Sequence domains: RNA recognition motif
Programmed cell death protein 7 Chain: D
Zinc finger matrin-type protein 5 Chain: E
Small nuclear ribonucleoprotein Sm D1 Chain: h
Small nuclear ribonucleoprotein Sm D3 Chain: j
Small nuclear ribonucleoprotein-associated proteins B and B' Chain: k
Small nuclear ribonucleoprotein E Chain: l
Small nuclear ribonucleoprotein F Chain: m
Small nuclear ribonucleoprotein G Chain: n
U11/U12 small nuclear ribonucleoprotein 48 kDa protein Chain: F
Small nuclear ribonucleoprotein Sm D2 Chain: i
P120-5'SS Chain: G
U11 snRNA Chain: R

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.5Å
Relevant EMDB volumes: EMD-51223
Expression system: Homo sapiens

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