9ge3

Electron Microscopy
2.87Å resolution

Structure of GPR55 in complex with G13 and endogenous lipid agonist lysophosphatidylinositol

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-287802 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein subunit alpha-13 Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 26.57 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14344 (Residues: 31-73, 203-377; Coverage: 55%)
  • Best match: Q14344-2 (Residues: 60-99, 100-100, 108-282)
Gene name: GNA13
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Single-chain variable fragment ScFv16 Chain: F
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
G-protein coupled receptor 55; Green fluorescent protein Chain: R

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.87Å
Relevant EMDB volumes: EMD-51288
Expression system: Trichoplusia ni