9gux

Electron Microscopy
3.3Å resolution

30S-TEC (TEC in expressome position) Inactive state 1

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 31-mer (preferred)
PDBe Complex ID:
PDB-CPX-283135 (preferred)
Entry contents:
26 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct RNA molecules
Macromolecules (30 distinct):
Small ribosomal subunit protein bS1 Chain: B
Molecule details ›
Chain: B
Length: 557 amino acids
Theoretical weight: 61.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0AG67 (Residues: 1-557; Coverage: 100%)
Gene names: JW0894, b0911, rpsA, ssyF
Sequence domains: S1 RNA binding domain
Small ribosomal subunit protein uS2 Chain: C
Molecule details ›
Chain: C
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A7V0 (Residues: 1-241; Coverage: 100%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: D
Small ribosomal subunit protein uS4 Chain: E
Small ribosomal subunit protein uS5 Chain: F
Small ribosomal subunit protein bS6 Chain: G
Small ribosomal subunit protein uS7 Chain: H
Small ribosomal subunit protein uS8 Chain: I
Small ribosomal subunit protein uS9 Chain: J
Small ribosomal subunit protein uS10 Chain: K
Small ribosomal subunit protein uS11 Chain: L
Small ribosomal subunit protein uS12 Chain: M
Small ribosomal subunit protein uS13 Chain: N
Small ribosomal subunit protein uS14 Chain: O
Small ribosomal subunit protein uS15 Chain: P
Small ribosomal subunit protein bS16 Chain: Q
Small ribosomal subunit protein uS17 Chain: R
Small ribosomal subunit protein bS18 Chain: S
Small ribosomal subunit protein uS19 Chain: T
Small ribosomal subunit protein bS20 Chain: U
Small ribosomal subunit protein bS21 Chain: V
DNA-directed RNA polymerase subunit alpha Chains: 1, 2
DNA-directed RNA polymerase subunit beta Chain: 3
DNA-directed RNA polymerase subunit beta' Chain: 4
DNA-directed RNA polymerase subunit omega Chain: 5
Transcription termination/antitermination protein NusG Chain: Z
Non-Template DNA strand Chain: 6
Template DNA strand Chain: 7
16S ribosomal RNA Chain: A
Messenger RNA Chain: X

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-51623
Expression system: Escherichia coli K-12