› 9ihs

X-ray diffraction
2Å resolution

Microbial transglutaminase mutant - D3C/G283C

Released:
DOI: 10.2210/pdb9ihs/pdb
PDB entry 9ihs coloured by chain, front view.
PDB entry 9ihs coloured by chain, side view.
PDB entry 9ihs coloured by chain, top view.
Source organism: Streptomyces mobaraensis
Primary publication:
Random mutagenesis and disulfide bond formation improved thermostability in microbial transglutaminase.
Suzuki M, Date M, Kashiwagi T, Takahashi K, Nakamura A, Tanokura M, Suzuki E, Yokoyama K
Appl. Microbiol. Biotechnol. (2024)

Function and Biology Details

Reaction catalysed: 
A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160522 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein-glutamine gamma-glutamyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 331 amino acids
Theoretical weight: 37.95 KDa
Source organism: Streptomyces mobaraensis
Expression system: Corynebacterium glutamicum
UniProt:
  • Canonical: P81453 (Residues: 77-407; Coverage: 88%)
Sequence domains: Microbial transglutaminase

Ligands and Environments

3 bound ligands:
Ligand MES 4 x MES
Ligand CL 4 x CL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 78.76Å b: 117.36Å c: 85.47Å
α: 90° β: 113.028° γ: 90°
R-values:
R R work R free
0.183 0.181 0.234
Expression system: Corynebacterium glutamicum

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