› 9ikq

X-ray diffraction
1.93Å resolution

Crystal structure of OPTN LZD in complex with GTP-bound Rab8a(Q67L)

Released:
DOI: 10.2210/pdb9ikq/pdb
Model geometry
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PDB entry 9ikq coloured by chain, front view.
PDB entry 9ikq coloured by chain, side view.
PDB entry 9ikq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular Basis of the Recognition of the Active Rab8a by Optineurin.
Zhang J, Liu L, Li M, Liu H, Gong X, Tang Y, Zhang Y, Zhou X, Lin Z, Guo H, Pan L
J. Mol. Biol. 436 168811-168811 (2024)
PMID: 39374890

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-283265 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-8A Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 20.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61006 (Residues: 1-181; Coverage: 87%)
Gene names: MEL, RAB8, RAB8A
Sequence domains: Ras family
Optineurin Chains: C, D
Molecule details ›
Chains: C, D
Length: 38 amino acids
Theoretical weight: 4.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96CV9 (Residues: 133-170; Coverage: 7%)
Gene names: FIP2, GLC1E, HIP7, HYPL, NRP, OPTN

Ligands and Environments

4 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand EDO 6 x EDO
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 67.218Å b: 69.432Å c: 110.337Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.218
Expression system: Escherichia coli

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