› 9ir4

Electron Microscopy
3.01Å resolution

Cryo-EM structure of Nipah virus L-P (H1165Y) polymerase complex

Released:
DOI: 10.2210/pdb9ir4/pdb
PDB entry 9ir4 coloured by chain, front view.
PDB entry 9ir4 coloured by chain, side view.
PDB entry 9ir4 coloured by chain, top view.
Source organism: Henipavirus nipahense
Primary publication:
Cryo-EM structure of Nipah virus L-P polymerase complex.
Peng Q, Dong Y, Jia M, Liu Q, Bi Y, Qi J, Shi Y
Nat Commun 15 10524 (2024)
PMID: 39627254
Related structures: EMD-60800

Function and Biology Details

Reactions catalysed: 
(1a) S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] = S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
(1a) (5')pppAACA-[mRNA] + (protein L)-L-histidine = diphosphate + (protein L)-L-histidyl-(5')phosphonato-AACA-[mRNA] + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-282583 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 2244 amino acids
Theoretical weight: 257.59 KDa
Source organism: Henipavirus nipahense
Expression system: Spodoptera
UniProt:
  • Canonical: Q997F0 (Residues: 1-2244; Coverage: 100%)
Gene name: L
Sequence domains:
Phosphoprotein Chains: C, D, E, F, G
Molecule details ›
Chains: C, D, E, F, G
Length: 709 amino acids
Theoretical weight: 78.39 KDa
Source organism: Henipavirus nipahense
Expression system: Spodoptera
UniProt:
  • Canonical: Q9IK91 (Residues: 1-709; Coverage: 100%)
Gene name: P/V/C
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.01Å
Relevant EMDB volumes: EMD-60800
Expression system: Spodoptera

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