› 9iy2

X-ray diffraction
3.48Å resolution

Immune complex of HEV-E2s, nAb 8C11 and nAb 8H3

Released:
DOI: 10.2210/pdb9iy2/pdb
PDB entry 9iy2 coloured by chain, front view.
PDB entry 9iy2 coloured by chain, side view.
PDB entry 9iy2 coloured by chain, top view.
Source organisms:
Entry authors: Minghua Z, Lizhi Z, Ying G, Shaowei L

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-250790 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Secreted protein ORF2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 23.12 KDa
Source organism: Hepatitis E virus (strain Pakistan)
Expression system: Bacterial expression vector pET-11a
UniProt:
  • Canonical: P33426 (Residues: 394-603; Coverage: 33%)
Gene name: ORF2
Sequence domains:
Heavy Chain of mAb 8C11 Chains: D, H
Molecule details ›
Chains: D, H
Length: 230 amino acids
Theoretical weight: 24.48 KDa
Source organism: Mus sp.
Light Chain of mAb 8C11 Chains: C, L
Molecule details ›
Chains: C, L
Length: 214 amino acids
Theoretical weight: 23.65 KDa
Source organism: Mus sp.
Heavy Chain of mAb 8H3 Chains: E, G
Molecule details ›
Chains: E, G
Length: 221 amino acids
Theoretical weight: 23.6 KDa
Source organism: Mus sp.
Light Chain of mAb 8H3 Chains: F, I
Molecule details ›
Chains: F, I
Length: 219 amino acids
Theoretical weight: 24.24 KDa
Source organism: Mus sp.

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 71.465Å b: 248.941Å c: 76.841Å
α: 90° β: 94.07° γ: 90°
R-values:
R R work R free
0.226 0.223 0.269
Expression system: Bacterial expression vector pET-11a

Quick links