› 9j0v

X-ray diffraction
1.79Å resolution

Crystal structure of monomeric PLP-dependent transaminase from Desulfobacula toluolica in P 21 21 21 space group

Released:
DOI: 10.2210/pdb9j0v/pdb
PDB entry 9j0v coloured by chain, front view.
PDB entry 9j0v coloured by chain, side view.
PDB entry 9j0v coloured by chain, top view.
Source organism: Desulfobacula toluolica
Entry authors: Matyuta IO, Bakunova AK, Nikolaeva AY, Rakitina TV, Bezsudnova EY, Popov VO, Boyko KM

Function and Biology Details

Reaction catalysed: 
D-alanine + 2-oxoglutarate = pyruvate + D-glutamate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-516459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dat: predicted D-alanine aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 32.06 KDa
Source organism: Desulfobacula toluolica
Expression system: Escherichia coli
UniProt:
  • Canonical: K0NPP0 (Residues: 1-286; Coverage: 100%)
Gene names: TOL2_C39420, dat
Sequence domains: Amino-transferase class IV

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand PEG 1 x PEG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 37.847Å b: 56.226Å c: 118.625Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.236
Expression system: Escherichia coli

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