9jm0

Electron Microscopy
2.7Å resolution

retron Ec86-effector fiber

Released:
Source organism: Escherichia coli
Related structures: EMD-61595

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-282727 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
2 distinct RNA molecules
Macromolecules (5 distinct):
Retron Ec86 reverse transcriptase Chains: A, B, K, P
Molecule details ›
Chains: A, B, K, P
Length: 330 amino acids
Theoretical weight: 37.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23070 (Residues: 1-320; Coverage: 100%)
Gene names: LM2_00877, ret
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)
Retron Ec86 putative ribosyltransferase/DNA-binding protein Chains: C, J, L, T
Molecule details ›
Chains: C, J, L, T
Length: 307 amino acids
Theoretical weight: 35.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DV88 (Residues: 1-307; Coverage: 100%)
Gene name: LM2_00875
DNA (85-MER) Chains: D, G, M, Q
RNA (82-MER) Chains: E, H, N, R
RNA (5'-R(P*CP*GP*UP*AP*AP*GP*GP*GP*UP*GP*CP*GP*CP*A)-3') Chains: F, I, O, S

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-61595
Expression system: Escherichia coli BL21(DE3)