9mhg

Electron Microscopy
3.2Å resolution

Cryo EM reconstruction of PI3KC3-C1 in complex with Human RAB1A(Q70L), VPS34 kinase domain in the inactive conformation

Released:
Source organism: Homo sapiens
Entry authors: Cook ASI, Hurley JH, Chen M
Related structures: EMD-48277

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-285236 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Phosphoinositide 3-kinase regulatory subunit 4 Chain: A
Molecule details ›
Chain: A
Length: 1409 amino acids
Theoretical weight: 158.81 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q99570 (Residues: 1-1358; Coverage: 100%)
Gene names: PIK3R4, VPS15
Sequence domains:
Phosphatidylinositol 3-kinase catalytic subunit type 3 Chain: B
Molecule details ›
Chain: B
Length: 887 amino acids
Theoretical weight: 101.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NEB9 (Residues: 1-887; Coverage: 100%)
Gene names: PIK3C3, VPS34
Sequence domains:
Beclin 1-associated autophagy-related key regulator Chain: C
Beclin-1 Chain: D
Ras-related protein Rab-1A Chain: E

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-48277
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21