All structure family : Transferase Inhibitor Protein From Tn5; Chain
 
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Entry Information
Entry status  (1)
REL
(6)
 
Experimental methods  (1)
X-ray diffraction
(6)
 
Authors  (15)
Rayment I
(6)
Reznikoff WS
(5)
Lovell S
(4)
Goryshin IY
(3)
Davies DR
(2)
Holden HM
(2)
Klenchin VA
(2)
Steiniger-White M
(2)
Thoden JB
(2)
Bhasin A
(1)
Braam LM
(1)
Czyz A
(1)
Gradman R
(1)
Mahnke Braam L
(1)
Reznikoff WR
(1)
 
Homo / hetero assembly  (2)
hetero
(5)
homo
(1)
 
Assembly composition  (2)
DNA/protein complex
(5)
protein structure
(1)
 
Assembly polymer count  (2)
hexamer
(5)
dimer
(1)
 
Resolution distribution
1.5 - 2
(1)
2.0 - 2.5
(3)
2.5 - 3
(2)
 
Release year distribution
1995 - 2000
(1)
2000 - 2005
(3)
2005 - 2010
(1)
2010 - 2015
(1)
 
Journal  (6)
Curr Opin Struct Biol
(1)
J Biol Chem
(1)
J Mol Biol
(1)
Nat Struct Biol
(1)
Nucleic Acids Res
(1)
Science
(1)
 
Macromolecules
Organism superkingdom  (1)
Bacteria
(6)
 
Organism name  (1)
Escherichia coli
(6)
 
Molecule name  (2)
Tnp
(6)
Transposase for transposon Tn5
(6)
 
Molecule type  (1)
Protein
(6)
 
Gene names  (2)
tnp
(6)
tnpA
(6)
 
Interacting Molecules  (6)
DNA NON-TRANSFERRED STRAND
(2)
DNA TRANSFERRED STRAND
(2)
DNA non-transferred strand
(2)
DNA transferred strand
(2)
ME DNA non-transferred strand
(1)
ME DNA transferred strand
(1)
 
Interacting ligands  (5)
MN : MANGANESE (II) ION
(4)
EDO : 1,2-ETHANEDIOL
(2)
MG : MAGNESIUM ION
(2)
GOL : GLYCEROL
(1)
TPT : 2,2':6',2''-TERPYRIDINE PLATINUM(II) Chloride
(1)
 
Function and Biology
Biological function  (4)
DNA binding
(6)
transposase activity
(6)
endonuclease activity
(5)
metal ion binding
(5)
 
Biological process  (3)
DNA transposition
(6)
DNA recombination
(5)
transposition
(5)
 
Sequence and Structure classification
SCOP fold  (1)
Ribonuclease H-like motif
(5)
 
SCOP family  (1)
Transposase inhibitor (Tn5 transposase)
(5)
 
CATH class  (2)
Alpha Beta
(6)
Mainly Alpha
(6)
 
CATH topology  (3)
Transferase Inhibitor Protein From Tn5; Chain A, domain2
(6)
Transposase Inhibitor Protein From Tn5; Chain A, domain 1
(6)
Serum Albumin; Chain A, Domain 1
(5)
 
Pfam accession / name  (2)
PF01609 : DDE_Tnp_1
(6)
PF14706 : Tnp_DNA_bind
(5)
 
Experimental Information
Diffraction protocol  (2)
Single wavelength
(4)
MAD
(2)
 
Diffraction radiation source type  (1)
Synchrotron
(6)
 
Diffraction source  (3)
APS BEAMLINE 19-ID
(4)
APS BEAMLINE 14-BM-D
(1)
APS BEAMLINE 19-BM
(1)
 
Synchrotron site  (1)
APS
(6)
 
Diffraction detector type  (1)
CCD
(6)
 
Refinement software  (3)
TNT
(3)
CNS
(2)
REFMAC
(2)
 
Representative Structures
Representative Structures
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Entries 1 to 6 of 6
Entries 1 to 6 of 6
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TRANSPOSASE INHIBITOR
Davies DR, Braam LM, Reznikoff WS, Rayment I
J Biol Chem (1999) [PMID: 10207011  ]
Source organism: Escherichia coli  
Assembly composition: protein only structure
Bound ligands: TPT   
Assembly name: Transposase for transposon Tn5 (Preferred)   search this complex
PDBe complex ID: PDB-CPX-175136 (Preferred)   search this ID
PDBe-KB: Q46731   
X-ray diffraction
2.9Å resolution
Released: 21 Apr 1999
Model geometry
Fit model/data
Data not deposited
1b7e
1b7e
1b7e
TN5 transposase: 20MER OUTSIDE END 2 MN complex
Klenchin VA, Lovell S, Goryshin IY, Reznikoff WR, Rayment I
Nat Struct Biol (2002) [PMID: 11896402  ]
Source organisms: Synthetic construct, Escherichia coli  
Assembly composition: DNA/protein complex
Bound ligands: MN    MN    EDO   
Assembly name: Transposase for transposon Tn5 and DNA (Preferred)   search this complex
PDBe complex ID: PDB-CPX-117331 (Preferred)   search this ID
PDBe-KB: Q46731   
X-ray diffraction
2.5Å resolution
Released: 22 Feb 2012
Model geometry
Fit model/data
4dm0
4dm0
4dm0
Crystal Structure Of Tn5 Transposase Complexed With 5' Phosphorylated Transposon End DNA
Klenchin VA
Nucleic Acids Res (2008) [PMID: 18790806  ]
Source organisms: Synthetic construct, Escherichia coli  
Assembly composition: DNA/protein complex
Bound ligands: GOL   
Assembly name: Transposase for transposon Tn5 and DNA (Preferred)   search this complex
PDBe complex ID: PDB-CPX-116204 (Preferred)   search this ID
PDBe-KB: Q46731    Q46731   
X-ray diffraction
2.5Å resolution
Released: 21 Oct 2008
Model geometry
Fit model/data
3ecp
3ecp
3ecp
crystal structure of Tn5 transposase complexed with resolved outside end DNA
Holden HM, Thoden JB, Steiniger-White M, Reznikoff WS, Lovell S, Rayment I
Curr Opin Struct Biol (2004) [PMID: 15102449  ]
Source organisms: Synthetic construct, Escherichia coli  
Assembly composition: DNA/protein complex
Bound ligands: MG    MN    MG    MN    EDO   
Assembly name: Transposase for transposon Tn5 and DNA (Preferred)   search this complex
PDBe complex ID: PDB-CPX-114206 (Preferred)   search this ID
PDBe-KB: Q46731   
X-ray diffraction
1.9Å resolution
Released: 27 Sep 2002
Model geometry
Fit model/data
1mus
1mus
1mus
CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH TRANSPOSON END DNA
Thoden JB, Holden HM, Davies DR, Goryshin IY, Reznikoff WS, Rayment I
Science (2000) [PMID: 10884228  ]
Source organisms: Synthetic construct, Escherichia coli  
Assembly composition: DNA/protein complex
Bound ligands: MG    MN    MG    MN   
Assembly name: Transposase for transposon Tn5 and DNA (Preferred)   search this complex
PDBe complex ID: PDB-CPX-114205 (Preferred)   search this ID
PDBe-KB: Q46731    Q46731   
X-ray diffraction
2.3Å resolution
Released: 27 Sep 2002
Model geometry
Fit model/data
1muh
1muh
1muh
Crystal structure of Tn5 Transposase complexed with ME DNA
Steiniger-White M, Bhasin A, Lovell S, Rayment I, Reznikoff WS
J Mol Biol (2002) [PMID: 12367522  ]
Source organisms: Synthetic construct, Escherichia coli  
Assembly composition: DNA/protein complex
Bound ligands: MN    MN   
Assembly name: Transposase for transposon Tn5 and DNA (Preferred)   search this complex
PDBe complex ID: PDB-CPX-114192 (Preferred)   search this ID
PDBe-KB: Q46731   
X-ray diffraction
2.8Å resolution
Released: 13 Dec 2002
Model geometry
Fit model/data
Data not deposited
1mm8
1mm8
1mm8
Entries 1 to 6 of 6
Entries 1 to 6 of 6