Uniprot accession / id : Q3UCV8  OR   Uniprot accession / id : Q96BN8
 
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revised
(1)
 
Entry Information
Entry status  (1)
REL
(11)
 
Experimental methods  (1)
X-ray diffraction
(11)
 
Authors  (53)
Elliott PR
(7)
Komander D
(7)
Keusekotten K
(5)
Krappmann D
(5)
Damgaard RB
(4)
Fiil BK
(4)
Gyrd-Hansen M
(4)
Almeida SM
(3)
Ceccarelli DF
(3)
Cordes SP
(3)
Dunham WH
(3)
Fukumura R
(3)
Gingras AC
(3)
Glockner L
(3)
Gondo Y
(3)
Hofmann K
(3)
Hospenthal MK
(3)
Huang H
(3)
Juang Y-C
(3)
Juang YC
(3)
Kulathu Y
(3)
MacLean TA
(3)
Raught B
(3)
Rivkin E
(3)
Sicheri F
(3)
Srikumar T
(3)
Wauer T
(3)
Xie G
(3)
Bonham S
(2)
El Oualid F
(2)
Kessler BM
(2)
Pinto-Fernandez A
(2)
Akutsu M
(1)
Berkun Y
(1)
Dikic I
(1)
Elpeleg O
(1)
Freund SM
(1)
Gomes LC
(1)
Harrison L
(1)
Kawasaki M
(1)
Kutzner K
(1)
Maher ER
(1)
Mailand N
(1)
Marco-Casanova P
(1)
Nielsen SV
(1)
Olma MH
(1)
Pfluger PT
(1)
Schaeffer V
(1)
Schaub A
(1)
Stangl A
(1)
Stepensky P
(1)
Swatek KN
(1)
Weber A
(1)
 
Homo / hetero assembly  (2)
hetero
(7)
homo
(4)
 
Assembly composition  (2)
protein/protein complex
(7)
protein structure
(4)
 
Assembly polymer count  (2)
dimer
(7)
monomer
(4)
 
Resolution distribution
1.0 - 1.5
(2)
1.5 - 2
(4)
2.0 - 2.5
(2)
2.5 - 3
(3)
 
Release year distribution
2010 - 2015
(8)
2015 - 2020
(3)
 
Journal  (6)
Cell
(3)
Nature
(3)
Mol Cell
(2)
Cell Chem Biol
(1)
EMBO Mol Med
(1)
Nat Commun
(1)
 
Macromolecules
Organism superkingdom  (1)
Eukaryota
(11)
 
Organism name  (1)
Homo sapiens
(11)
 
Molecule name  (4)
Deubiquitinating enzyme otulin
(11)
OTU domain-containing deubiquitinase with linear linkage specificity
(11)
Ubiquitin thioesterase Gumby
(11)
Ubiquitin thioesterase otulin
(11)
 
Molecule type  (1)
Protein
(11)
 
Gene names  (2)
FAM105B
(11)
OTULIN
(11)
 
Interacting Molecules  (3)
Ubiquitin
(4)
E3 ubiquitin-protein ligase RNF31
(2)
Sorting nexin-27
(1)
 
Interacting ligands  (5)
GOL : GLYCEROL
(5)
CL : CHLORIDE ION
(3)
SO4 : SULFATE ION
(3)
CA : CALCIUM ION
(2)
BME : BETA-MERCAPTOETHANOL
(1)
 
Function and Biology
EC number / name  (1)
3.4.19.12 : Ubiquitinyl hydrolase 1
(11)
 
Biological function  (1)
cysteine-type deubiquitinase activity
(9)
 
Biological process  (1)
protein linear deubiquitination
(9)
 
Sequence and Structure classification
Pfam accession / name  (1)
PF16218 : Peptidase_C101
(9)
 
Experimental Information
Diffraction protocol  (1)
Single wavelength
(11)
 
Diffraction radiation source type  (2)
Synchrotron
(10)
Rotating anode
(1)
 
Diffraction source  (8)
APS BEAMLINE 24-ID-C
(2)
DIAMOND BEAMLINE I03
(2)
DIAMOND BEAMLINE I04
(2)
DIAMOND BEAMLINE I02
(1)
ESRF BEAMLINE ID14-4
(1)
ESRF BEAMLINE ID23-1
(1)
PHOTON FACTORY BEAMLINE BL-1A
(1)
RIGAKU MICROMAX-007 HF
(1)
 
Synchrotron site  (4)
Diamond
(5)
APS
(2)
ESRF
(2)
Photon Factory
(1)
 
Diffraction detector type  (2)
Pixel
(6)
CCD
(5)
 
Refinement software  (2)
PHENIX
(6)
REFMAC
(5)
 
Representative Structures
Representative Structures
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Entries 1 to 10 of 11
Entries 1 to 10 of 11
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Structure of OTULIN bound to the Met1-linked diubiquitin activity probe
Elliott PR, Komander D
Cell Chem Biol (2017) [PMID: 28919039  ]
Source organism: Homo sapiens  
Assembly composition: protein/protein complex
Modified residues: DHA   
Assembly name: Ubiquitin thioesterase otulin and Ubiquitin (Preferred)   search this complex
PDBe complex ID: PDB-CPX-143393 (Preferred)   search this ID
PDBe-KB: Q96BN8    P0CG48   
X-ray diffraction
2.95Å resolution
Released: 27 Sep 2017
Model geometry
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5oe7
5oe7
5oe7
Structure of the OTULINcat C129A - SNX27 PDZ domain complex.
Elliott PR, Komander D
Nat Commun (2019) [PMID: 31541095  ]
Source organism: Homo sapiens  
Assembly composition: protein/protein complex
Bound ligands: GOL    SO4   
Assembly name: Ubiquitin thioesterase otulin and Sorting nexin-27 (Preferred)   search this complex
PDBe complex ID: PDB-CPX-188380 (Preferred)   search this ID
PDBe-KB: Q96BN8    Q96L92   
X-ray diffraction
2Å resolution
Released: 2 Oct 2019
Model geometry
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6sak
6sak
6sak
Crystal structure of HOIP PUB domain in complex with OTULIN PIM
Akutsu M, Schaeffer V, Olma MH, Gomes LC, Kawasaki M, Dikic I
Mol Cell (2014) [PMID: 24726327  ]
Source organism: Homo sapiens  
Assembly composition: protein/protein complex
Assembly name: E3 ubiquitin-protein ligase RNF31 and Ubiquitin thioesterase otulin (Preferred)   search this complex
PDBe complex ID: PDB-CPX-188379 (Preferred)   search this ID
PDBe-KB: Q96BN8    Q96EP0   
X-ray diffraction
2.7005Å resolution
Released: 7 May 2014
Model geometry
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4p0b
4p0b
4p0b
Crystal structure of OTULIN OTU domain (C129A) in complex with Met1- di ubiquitin
Keusekotten K, Elliott PR, Glockner L, Kulathu Y, Wauer T, Krappmann D, Hofmann K, Komander D
Cell (2013) [PMID: 23746843  ]
Source organism: Homo sapiens  
Assembly composition: protein/protein complex
Bound ligands: SO4   
Assembly name: Ubiquitin thioesterase otulin and Ubiquitin (Preferred)   search this complex
PDBe complex ID: PDB-CPX-143393 (Preferred)   search this ID
PDBe-KB: Q96BN8    P0CG48   
X-ray diffraction
1.9Å resolution
Released: 26 Jun 2013
Model geometry
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3znz
3znz
3znz
Structure of the OTU domain of OTULIN G281R mutant
Damgaard RB, Elliott PR, Swatek KN, Maher ER, Stepensky P, Elpeleg O, Komander D, Berkun Y
EMBO Mol Med (2019) [PMID: 30804083  ]
Source organism: Homo sapiens  
Assembly composition: protein only structure
Bound ligands: GOL    CL   
Assembly name: Ubiquitin thioesterase otulin (Preferred)   search this complex
PDBe complex ID: PDB-CPX-188378 (Preferred)   search this ID
PDBe-KB: Q96BN8   
X-ray diffraction
1.77Å resolution
Released: 6 Mar 2019
Model geometry
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6i9c
6i9c
6i9c
Crystal structure of the OTU domain of OTULIN at 1.3 Angstroms.
Keusekotten K, Elliott PR, Glockner L, Kulathu Y, Wauer T, Krappmann D, Hofmann K, Komander D
Cell (2013) [PMID: 23746843  ]
Source organism: Homo sapiens  
Assembly composition: protein only structure
Bound ligands: CA    GOL    CA    CL   
Assembly name: Ubiquitin thioesterase otulin (Preferred)   search this complex
PDBe complex ID: PDB-CPX-188378 (Preferred)   search this ID
PDBe-KB: Q96BN8   
X-ray diffraction
1.3Å resolution
Released: 26 Jun 2013
Model geometry
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3znv
3znv
3znv
Structure of HOIP PUB domain bound to OTULIN PIM
Elliott PR, Komander D
Mol Cell (2014) [PMID: 24726323  ]
Source organism: Homo sapiens  
Assembly composition: protein/protein complex
Bound ligands: CL   
Assembly name: E3 ubiquitin-protein ligase RNF31 and Ubiquitin thioesterase otulin (Preferred)   search this complex
PDBe complex ID: PDB-CPX-188379 (Preferred)   search this ID
PDBe-KB: Q96BN8    Q96EP0   
X-ray diffraction
2.0001Å resolution
Released: 21 May 2014
Model geometry
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4oyk
4oyk
4oyk
Crystal structure of the OTU domain of Gumby/Fam105B at 1.6 angstrom
Ceccarelli DF, Juang Y-C, Sicheri F
Nature (2013) [PMID: 23708998  ]
Source organism: Homo sapiens  
Assembly composition: protein only structure
Bound ligands: GOL    BME   
Modified residues: MSE    MSE   
Assembly name: Ubiquitin thioesterase otulin (Preferred)   search this complex
PDBe complex ID: PDB-CPX-188378 (Preferred)   search this ID
PDBe-KB: Q96BN8   
X-ray diffraction
1.6Å resolution
Released: 5 Jun 2013
Model geometry
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4ksj
4ksj
4ksj
Gumby/Fam105B in complex with ubiquitin
Ceccarelli DF, Juang Y-C, Sicheri F
Nature (2013) [PMID: 23708998  ]
Source organism: Homo sapiens  
Assembly composition: protein/protein complex
Bound ligands: SO4   
Assembly name: Ubiquitin thioesterase otulin and Ubiquitin (Preferred)   search this complex
PDBe complex ID: PDB-CPX-143393 (Preferred)   search this ID
PDBe-KB: Q96BN8    P0CG48   
X-ray diffraction
2.4Å resolution
Released: 5 Jun 2013
Model geometry
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4ksk
4ksk
4ksk
Gumby/Fam105B in complex with linear di-ubiquitin
Juang Y-C, Ceccarelli DF, Sicheri F
Nature (2013) [PMID: 23708998  ]
Source organism: Homo sapiens  
Assembly composition: protein/protein complex
Assembly name: Ubiquitin thioesterase otulin and Ubiquitin (Preferred)   search this complex
PDBe complex ID: PDB-CPX-143393 (Preferred)   search this ID
PDBe-KB: Q96BN8    P0CG48   
X-ray diffraction
2.83Å resolution
Released: 5 Jun 2013
Model geometry
Fit model/data
Data not analysed
4ksl
4ksl
4ksl
Entries 1 to 10 of 11
Entries 1 to 10 of 11