PDBsum entry 3ljq

Hydrolase

PDB id: 3ljq

Contents

Protein chains

281 a.a. *

Ligands

GLY

Metals

_NA ×2

Waters ×512

* Residue conservation analysis

PDB id:

3ljq

Name:

Hydrolase

Title:

Crystal structure of the glycosylasparaginase t152c apo-precursor

Structure:

N(4)-(beta-n-acetylglucosaminyl)-l-asparaginase. Chain: a, c. Fragment: unp residues 46-340. Synonym: glycosylasparaginase, aspartylglucosaminidase, aga, n4-(n- acetyl-beta-glucosaminyl)-l-asparagine amidase, glycosylasparaginase alpha chain, glycosylasparaginase beta chain. Engineered: yes. Mutation: yes

Source:

Flavobacterium meningosepticum. Organism_taxid: 238. Gene: ga(1-295). Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.90Å R-factor: 0.154 R-free: 0.197

Authors:

Y.Wang,H.-C.Guo

Key ref:

Y.Wang and H.C.Guo (2010). Crystallographic snapshot of glycosylasparaginase precursor poised for autoprocessing. J Mol Biol, 403, 120-130. PubMed id: 20800597

Date:

26-Jan-10 Release date: 24-Nov-10

Protein chains

Q47898  (ASPG_ELIMR) -  N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase from Elizabethkingia miricola

Seq: 340 a.a.

Struc: 281 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.5.1.26 - N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase.
• Reaction: N₄-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O = N-acetyl-beta-D- glucosaminylamine + L-aspartate + H⁺

N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O = N-acetyl-beta-D- glucosaminylamine + L-aspartate (Bound ligand (Het Group name = GLY) matches with 55.56% similarity) + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Mol Biol 403:120-130 (2010)

PubMed id: 20800597

Crystallographic snapshot of glycosylasparaginase precursor poised for autoprocessing.

Y.Wang, H.C.Guo.

ABSTRACT

No abstract given.