Virtual course
CABANA workshop: Analysis of crop genomics data
Please note this course was postponed in 2020 due to the COVID-19 pandemic and will take place virtually in 2021.
This course will introduce crop biologists to methods and approaches for analysing crop genomics data for genotyping. During the course participants will learn about genotyping experimental design, variant calling, comparative genomics using Ensembl, analyse variation data with Ensembl Variant Effect Predictor and the European Variation Archive and explore concepts of genome wide association studies (GWAS). The content will explain how to make use of existing public data, retrieve, submit and share your data with the community.
It will also provide training on using R in genomics applications for crop genotyping. Trainers will cover the basics of diversity analysis using R, before analyzing various genomes to assess diversity. This will include hierarchical clustering, correlating genomic data with geographic and phenotypic information and investigating gene-phenotype relationships and microsatellites. This course will use genomic data of wheat, maize and potato as training datasets throughout the week. The course will be delivered through a combination of hands-on training sessions and lectures.
Please note: The course will use genomic data from crop plant species throughout the week. It will not explore in detail methods for assembling genomes.
Who is this course for?
This course is aimed at researchers at graduate level upwards within Latin America who are working with and/or generating their own crop genomic data or who are working on genotyping technologies, including data analysis software development.
Prerequisites: Basic computational or previous bioinformatics experience is required for this workshop, such as basic commands in R. Undergraduate-level knowledge of plant biology would be an advantage.
Please note this course will be taught in English. There will be assistants fluent in Spanish who can offer language support where feasible.
What will I learn?
Learning outcomes
By the end of this course, participants should be able to:
- Describe how to design a crop genotyping experiment
- Conduct variant calling and search for a variety of crop genomic motifs such as SSRs and indels
- Use Ensembl for comparative analysis to explore plant genomes
- Access plant variation data from public resources and use appropriate tools to analyse variation
- Input and study genomics data using R to evaluate genomic diversity
- Select the best method to investigate genomic diversity
Course content
During this course you will learn about:
- How to design sequence-based genotyping experiments and conduct variant calling
- Ensembl tools for browsing plant genomes and variant data
- Using R to assess crop diversity
- Genome wide association studies (GWAS)
Trainers
Marco Cristancho
Universidad de Los Andes, Colombia Diana Lopez
Universidad Nacional de Colombia, Colombia Carla Valeria Filippi
EMBL-EBI, UK Bruno Contreras-Moreira
EMBL-EBI, UK Manuel Humberto Reyes-Valdes
Universidad Autónoma Agraria Antonio Narro (UAAAN), Mexico Octavio Martinez de la Vega
LANGEBIO, Cinvestav, Mexico
Universidad de Los Andes, Colombia
Universidad Nacional de Colombia, Colombia
EMBL-EBI, UK
EMBL-EBI, UK
Universidad Autónoma Agraria Antonio Narro (UAAAN), Mexico
LANGEBIO, Cinvestav, Mexico
Programme
Day 1 – Monday 1st March 2021 |
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08:45-09:00 | Arrival | |
09:00-09:30 | Welcome and introduction | Marco Cristancho / Diana López |
09:30-10:30 | Methodologies for genotyping and NGS overview | Diana López |
10:30-11:00 | Break | |
11:00-11:45 | Introduction to variant calling | Diana López |
11:45-12:30 | Planning a genotyping experiment | Diana López |
12:30 | End of day 1 | |
Day 2 – Tuesday 2nd March 2021 |
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08:00-09:00 | Whole genome variant calling: Strategies for SNP, small indels and SSR whole genome calling | Carla Filippi |
09:00-10:00 | Structural Variant calling: Large insertions, deletions, translocations | Carla Filippi |
10:00-10:30 | Break | |
10:30-11:45 | Introduction to the Ensembl Plants browser | Bruno Contreras |
11:45-12:45 | Trainee presentations | Trainees |
12:45 | End of day 2 |
Day 3 – Wednesday 3rd March 2021 |
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08:30-10:00 | VEP and Expression Atlas | Bruno Contreras |
10:00-10:30 | Break | |
10:30-12:00 | Retrieving data from Ensembl Compara | Bruno Contreras |
12:00-12:30 | Interacting with Ensembl: requesting new genomes, annotations or variants | Bruno Contreras |
12:30 | End of day 3 | |
Day 4 – Thursday 4th March 2021 |
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09:00-10:00 | Pan-genome Analysis | Bruno Contreras |
10:00-10:30 | Break | |
10:30-11:45 | Pan-genome Analysis | Bruno Contreras |
11:45-12:45 | Trainee presentations | Trainees |
12:45 | End of day 4 | |
Day 5 – Friday 5th March 2021 |
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08:30-10:00 | R-genes identification and characterisation | Carla Filippi |
10:00-10:30 | Break | |
10:30-12:00 | Overview of R | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
12:00 | End of day 5 | |
Day 6 – Monday 8th March 2021 |
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08:30-10:00 | Genomic Molecular Markers | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
10:00-11:15 | Measuring diversity | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
11:15-11:45 | Break | |
11:45-12:15 | Wheat data from CIMMYT | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
12:15-13:15 | Assessing and summarizing diversity in wheat data | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
13:15-14:15 | Trainee presentations | Trainees |
14:15 | End of day 6 | |
Day 7 – Tuesday 9th March 2021 |
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09:00-09:30 | Maize microsatellites | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
09:30-10:30 | Assessing diversity in maize microsatellites | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
10:30-11:00 | Break | |
11:00-11:30 | Summarizing diversity in maize microsatellites | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
11:30-12:30 | Testing diversity in maize microsatellites | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
12:30 | End of day 7 | |
Day 8 – Wednesday 10th March 2021 |
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09:00-10:30 | Core Subset selection: concepts | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
10:30-11:00 | Break | |
11:00-12:30 | Core Subset selection in maize and wheat | Octavio Martinez de la Vega/Manuel Humberto Reyes-Valdés |
12:30-13:30 | Trainee presentations | Trainees |
13:30 | End of day 8 | |
Day 9 – Thursday 11th March 2021 |
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09:00-10:00 | Introduction to genome wide association studies (GWAS) | Diana López |
10:00-10:15 | Break | |
10:15-11:45 | Practical: GWAS in diploid crops data | Diana López |
11:45-12:15 | Q&A on Molecular markers | Diana López |
12:15-12:30 | Wrap-up and feedback | Marco Cristancho |
12:30 | End of course |
Please note this course was postponed in 2020 due to the COVID-19 pandemic and will take place virtually in 2021.
In order to be considered for a place on this course applicants must complete the online application form.
Incomplete applications will NOT be considered.
If you have any queries about the registration process please email Guilherme Oliveira and Piv Gopalasingam.
There will be a maximum of 30 participants on this course and registration is dependent on selection after successful completion of the application process
Applications will close on 9th February 2020 at 23:30 (GMT)