Virtual course
CABANA Workshop: Genomic and transcriptomic resources for eukaryotic species
This course is intended to be a gentle introduction to the computational resources available for analysis of omics data in eukaryotic species. Specifically, we will cover: 1) an optional Linux crash course; 2) file formats commonly used in NGS data analysis; 3) web resources available for the analysis of eukaryotic omics data; 4) common bioinformatics pipelines used to analyse genomic/transcriptomic data from eukaryotic species (sequence alignment, phylogenetic tree reconstruction, variant detection, RNA-Seq and data visualization in genome browsers). Live components of the course will run around 09:00-12:00 BRT and you are encouraged to use the afternoon to practice, using a specified forum or communication method to ask for support. Support will end around 17:00 BRT each day.
Who is this course for?
This course is suitable for postgraduate students (MSc-level and above), postdoctoral-level researchers, newly established principal investigators and other research scientists within Latin America, who wish to use omics resources/analysis in their own research. We welcome applications from universities, research centres, hospitals, public health, and private laboratories.
Scientists from underrepresented ethnic and gender groups are especially encouraged to apply for this workshop, for example women and those with Black and/or Indigenous heritage.
Please note this course will be taught in English, however the trainers are fluent in either Portuguese or Spanish and can offer language support where feasible. Attendance priority will be given to those who have not attended a CABANA event yet.
Prerequisites
Working knowledge of genetics and/or molecular biology would be advantageous. You will also require access to a computer for the duration of the workshop. You do not need to know how to use UNIX / Linux operating systems and the command line, as the course covers the basics.
What will I learn?
Learning outcomes
By the end of this course you should be able to:
- Know how to use available web resources to search and analyze genomic/transcriptomic data from eukaryotic species.
- List the most common file formats used in contemporary "omics" research.
- Use a Linux terminal to execute bioinformatics pipelines for common tasks, such as variant detection, sequence alignment, and phylogenetic reconstruction
Trainers
UFMG - Universidade Federal de Minas Gerais
UFRJ - Universidade Federal de Rio de Janeiro
Programme
Time |
Subject |
Trainer(s) |
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Day 1 – Monday 2nd August 2021 |
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09:00-09:30 |
Welcome, introduction, expectations and networking |
All/Piv/Francisco |
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09:30-10:30 |
Introduction to CABANA, EMBL-EBI, resources and tools. |
Piv Gopalasingam |
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10:30-11:00 |
Coffee Break |
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11:00-12:00 |
Genome projects and DNA sequencing technologies - history and applications (lecture) |
Francisco Prosdocimi |
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Day 2 – August 3 2021 |
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09:00-9:30 |
Linux command line short crash course (lecture/practical demonstration) |
Francisco Lobo |
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9:30-10:00 |
Quality control in NGS data (lecture/practical demonstration) |
Francisco Lobo |
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10:30-11:00 |
Coffee Break |
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11:00-12:00 |
Read mapping in reference genomes for variant detection (lecture/practical demonstration) |
Francisco Lobo |
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Day 3 – August 4 2021 |
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09:00-10:00 |
BAM file manipulation (lecture/practical demonstration) |
Francisco Lobo |
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10:00-10:30 |
Sequence alignment (lecture/practical demonstration) |
Francisco Prosdocimi |
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10:30-11:00 |
Coffee Break |
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11:00-12:00 |
Phylogenetic tree reconstruction (lecture/practical demonstration) |
Francisco Prosdocimi |
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Day 4 – August 5 2021 |
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09:00-09:30 |
Variants in genomic data (lecture) |
Francisco Lobo |
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09:30-10:30 |
Variant calling/filtering (lecture/practical demonstration) |
Francisco Lobo |
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10:30-11:00 |
Coffee Break |
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11:00-12:00 |
Variant annotation (lecture/practical demonstration) |
Francisco Lobo |
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Day 5 – August 6 2021 |
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09:00-10:00 |
Experimental design for RNA-Seq (lecture) |
Adriano/Gabriel |
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10:00-11:00 |
Exploring gene expression data with Expression Atlas (lecture/practical) |
Adriano/Gabriel |
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11:00-11:30 |
Coffee Break |
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11:30-12:00 |
RNA-Seq analysis (lecture) |
Adriano/Gabriel |
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Day 6 – August 9 2021 |
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09:00-09:30 |
RNA-Seq analysis (practical demonstration) |
Adriano/Gabriel |
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09:30-10:30 |
Differential gene expression analysis (lecture/practical demonstration) |
Adriano/Gabriel |
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10:30-11:00 |
Coffee Break |
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11:00-12:00 |
Ensembl Genome Browser & other resources (lecture/practical demonstration) |
Mariana |
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Day 7 – August 10 2021 |
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09:00-10:00 |
Searching for data at EMBL-EBI (lecture/practical demonstration) |
Mariana/ Francisco Lobo |
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10:00-11:00 |
Course close and feedback |
All |
In order to be considered for a place on this course applicants must complete the online application form.
Incomplete applications will NOT be considered.
If you have any general queries about the application process please email Guilherme Oliveira and Piv Gopalasingam.
For workshop queries please email Francisco Lobo.
Please note that this course is free, but unexplained absence will result in blacklisting for future courses and opportunities.
There will be a maximum of 30 participants on this course and registration is dependent on selection after successful completion of the application process.
Applications will close on Sunday 18th July 2021 at 23:45 BRT (Brasilia time).