Course outside EMBL-EBI
CABANA Workshop: Introduction to Metagenomics
In this course participants will learn the basics of metagenomics, covering experimental design and workflows, moving through to microbiome analysis via metabarcoding and shotgun metagenomics. The course theme will be the use of metagenomics oriented to biodiversity.
The course will be delivered by experienced and dedicated world-renowned experts, including EMBL-EBI trainers, and will involve presentations, demonstrations and practical exercises to give participants hands-on experience.
Who is this course for?
This course is suitable for postgraduate wet-lab researchers and also bioinformaticians at graduate-level upwards within Latin America who are working with and/or generating their own metagenomics datasets.
Prerequisites:
Undergraduate-level knowledge of biology would be advantageous. Participants will also require a basic knowledge of the Unix command line, and the R statistical packages. We recommend these free tutorials:
- Basic introduction to the Unix environment: www.ee.surrey.ac.uk/Teaching/Unix
- Introduction and exercises for Linux: https://training.linuxfoundation.org/free-linux-training
- Basic R concept tutorials: www.r-tutor.com/r-introduction
Please note this course will be taught in English, however the trainers are fluent in either Spanish, and can offer language support where feasible.
A number of travel fellowships are available for this course - early-stage researchers and researchers from underrepresented groups are especially encouraged to apply for CABANA travel fellowships. You can apply for travel fellowships via the course application form.
What will I learn?
Learning outcomes
After this course you should be able to:
- Use Unix and R tools for metagenomics analyses
- Process metagenomics data
- Use metagenomics databases
- Annotate genome and metagenomes
- Analyse metagenomic results
Course content
During this course you will learn about:
- Basic Unix and R environment for metagenomics
- FastQC for Quality control and Trimmomatic for preprocessing
- MGnify annotation pipeline for Metabarcoding
- SPAdes and metaSPAdes for assembly, metaWRAP for binning
- InterProScan and Genome Properties for genome and metagenome annotation
- R tools for metagenomics analyses such as dada2, Phyloseq, ggplot2 and gggenes
Trainers
Valeria Faggioli
INTA EEA Marcos Juárez, Argentina Federico Vignale
FCEN-UBA, Argentina Jonathan Zaiat
IQUIBICEN, FCEN-UBA, Argentina Marcelo Marti
FCEN-UBA, Argentina Leonardo Erijman
FCEN-UBA, Argentina Adrian Turjanski
University of Buenos Aires, Argentina Lorna Richardson
EMBL-EBI, UK Alex Mitchell
EMBL-EBI, UK Alejandro Reyes
Universidad de los Andes, Colombia
INTA EEA Marcos Juárez, Argentina
FCEN-UBA, Argentina
IQUIBICEN, FCEN-UBA, Argentina
FCEN-UBA, Argentina
FCEN-UBA, Argentina
University of Buenos Aires, Argentina
EMBL-EBI, UK
EMBL-EBI, UK
Universidad de los Andes, Colombia
Programme
Day 1 – Monday 2 December 2019 |
||
---|---|---|
08:30-09:00 | Arrival and registration | |
09:00-10:00 | Welcome and introductions | Adrian Turjanski |
10:00-11:00 | Introduction to sequencing technologies | Adrian Turjanski |
11:00-11:30 | Break | |
11:30-13:00 | Introduction to genomics and metagenomics | Federico Vignale |
13:00-14:00 | Lunch | |
14:00-15:30 | Practical Session: Unix and R | Federico Vignale/Jonathan Zaiat |
15:30-16:00 | Break | |
16:00-17:30 | Practical Session: Quality control and pre-processing | Federico Vignale |
17:30 | End of day 1 |
Day 2 – Tuesday 3 December 2019 |
||
---|---|---|
09:00-11:00 | Introduction to metabarcoding | Alex Mitchell |
11:00-11:30 | Break | |
11:30-13:00 | MGnify | Alex Mitchell |
13:00-14:00 | Lunch | |
14:00-15:30 | Practical Session: Metabarcoding part 1 | Agustin Maria Pardo/Federico Vignale |
15:30-16:00 | Break | |
16:00-17:30 | Practical Session: Metabarcoding part 2 | Agustin Maria Pardo/Federico Vignale |
17:30 | End of day 2 | |
Day 3 – Wednesday 4 December 2019 |
||
09:00-11:00 | Genome and metagenome assembly | Alejandro Reyes |
11:00-11:30 | Break | |
11:30-13:00 | Metagenome-assembled genomes (MAGs) | Alex Mitchell |
13:00-14:00 | Lunch | |
14:00-15:30 | Practical Session: Assembly | Alejandro Reyes/Federico Vignale |
15:30-16:00 | Break | |
16:00-17:30 | Practical Session: Binning | Alex Mitchell/Federico Vignale |
17:30 | End of day 3 | |
Day 4 – Thursday 5 December 2019 |
||
09:00-10:00 | Introduction to databases | Marcelo Marti |
10:00-11:00 | InterProScan | Lorna Richardson |
11:00-11:30 | Break | |
11:00-13:00 | Genome Properties | Lorna Richardsoon |
13:00-14:00 | Lunch | |
14:00-15:30 | Practical Session: Genome and metagenome annotation part 1 | Lorna Richardson/Federico Vignale |
15:30-16:00 | Break | |
16:00-17:30 | Practical Session: Genome and metagenome annotation part 2 | Lorna Richardson/Federico Vignale |
17:30 | End of day 4 | |
Day 5 – Friday 6 December 2019 |
||
09:00-11:00 | Applications of metagenomics | Alejandro Reyes |
11:00-11:30 | Break | |
11:30-13:00 | Applications of metagenomics | Leonardo Erijman |
13:00-14:00 | Lunch | |
14:00-15:30 | Practical Session: Analysis of metagenomics results | Federico Vignale/Agustin Maria Pardo |
15:30-16:00 | Break | |
16:00-17:30 | Exam; Course close and feedback | Adrian Turjanski |
17:30 | End of course |
In order to be considered for a place on this course applicants must complete the online application form.
Incomplete applications will NOT be considered.
If you have any queries about the registration process please email Guilherme Oliveira and Piv Gopalasingam.
Please note that the registration fee of $1300 ARS covers:
- All meals during the course (lunch and refreshment breaks)
- Course materials
- Course dinner
There will be a maximum of 25 participants on this course and registration is dependent on selection after successful completion of the application process.