This course will provide an introduction to the technology, data analysis, tools and resources for next generation sequencing (NGS) data.

Topics will be delivered using a mixture of lectures, practical sessions and open discussions. Practical work during the course will use small, example data-sets and there will not be scope to analyse personal data. The course content makes use of data derived mainly from eukaryotic non-model organisms in a biodiversity context.

Who is this course for?

This course is suitable for postgraduate wet-lab researchers at any career stage within Latin America who are working with and/or generating their own transcriptomic datasets.
 
Participants will require a basic knowledge of the Unix command line and the R statistical package. We recommend these free tutorials:

• Basic introduction to the Unix environment: www.ee.surrey.ac.uk/Teaching/Unix
• Introduction and exercises for Bio-Linux 7: http://nebc.nerc.ac.uk/support/training/course-notes/past-notes/intro-bl7
• Basic R concept tutorials: www.r-tutor.com/r-introduction 

 Participants without basic knowledge of these resources will have difficulty in completing the practical sessions. We strongly recommend that you make every effort to access these materials and other training available online or within your host institute to best prepare you for the course.
 
A number of travel fellowships are available for this course - early-stage researchers and researchers from underrepresented groups are especially encouraged to apply for CABANA travel fellowships. You can apply for travel fellowships via the course application form.

What will I learn?

Learning outcomes

After this course you should be able to:

• Describe NGS and its applications in biological research
• Perform read alignments
• Assemble transcriptomes
• Perform differential gene expression analysis and visualize results in R and ggplot2 
• View and explore data using Ensembl genome browser

Course content

• Introduction to Ensembl/Ensembl Genomes

Trainers

In order to be considered for a place on this course applicants must complete the online application form.

Incomplete applications will NOT be considered. 

If you have any queries about the registration process please email Piv Gopalasingam.

The registration fee includes:

• Tea/coffee and lunches will be provided for the duration of the course
• Course dinner on Tuesday 19 March
• Use of the course training room
• Course materials provided
• Course certificate