Course at EMBL-EBI
Introduction to metabolomics analysis
This course will provide an introduction to metabolomics data analysis using publicly available software and tools. Participants will become familiar with the current state of experimental design, data standards, analysis and sharing in metabolomics, particularly through using the EMBL-EBI’s MetaboLights repository and PhenoMeNal infrastructure. There will be a practical component, where participants will learn through hands-on tutorials to use some of the tools available for data analysis and data submission. The course will be completed with an interactive session which will build on the weeks learning to discuss how to develop a metabolomics workflow with a clinical case study.
Who is this course for?
This course is aimed at researchers who are new to the field of metabolomics and wish to learn about the process of developing a metabolomics study. Attendees will gain an understanding of the workflow process and some of the analysis techniques and so should benefit those who are planning to include metabolite profiling in their work, either moving into the field or as an addition to other omics. We will primarily focus on a basic introduction to metabolomics with a worked example using a predesigned LC-MS analysis workflow. The course assumes little prior knowledge of using bioinformatics tools.
What will I learn?
Learning outcomes
After this course you should be able to:
- Discuss basic analytical methods in metabolomics, and factors that contribute to designing a successful metabolomics study
- Discuss the issues of data sharing and data standards in metabolomics
- Access metabolomics resources
- Be familiar with the principles of creating a standardized workflow to perform basic metabolomics analysis
Course content
During this course you will learn about:
- Basic metabolomics experimental design
- Case study based on a clinical example
- Metabolomics resources: MetaboLights, Phenomenal, MetaboFlow
- Open source metabolomics tools made available through customisable workflows.
Trainers
Alexandra Holinski
EMBL-EBI, UK Keeva Cochrane
EMBL-EBI, UK Claire O’Donovan
EMBL-EBI, UK Nicola Gray
University of Reading, UK Ralf Weber
University of Birmingham, UK Alyssa Imbert
CNRS, France Steffen Neumann
IPB, Halle, Germany MetaboLights team
EMBL-EBI, UK Kim Kultima
Uppsala University, Sweden
EMBL-EBI, UK
EMBL-EBI, UK
EMBL-EBI, UK
University of Reading, UK
University of Birmingham, UK
CNRS, France
IPB, Halle, Germany
EMBL-EBI, UK
Uppsala University, Sweden
Programme
Day 1 – Tuesday 5th February 2019 |
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10:30 | Registration & coffee | |
11:00 - 11:30 | Introduction to EMBL-EBI | Alexandra Holinski |
11:30 - 13:00 |
Part 1 Overview experimental design Experimental design - ensuring data quaility |
Nicola Gray |
13:00 - 14:00 | Lunch | |
14:00 - 15:30 | Part 1 Introduction to analytical platforms; overview all options; Introduction to LC-MS Hands on experimental design |
Nicola Gray |
15:30 - 16:00 | Tea/coffee break | |
16:00 - 17:00 | Part 2 Basics of metabolomics analysis; What is a workflow |
Ralf Weber |
18:30 | Evening meal |
Day 2 – Wednesday 6th February 2019 |
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09:30 - 10:30 | Part 3 Processing data | Ralf Weber |
10:30 - 11:00 | Tea/coffee break | |
11:00 - 13:00 | Part 3 Processing data | Ralf Weber |
13:00 - 14:00 | Lunch | |
14:00 - 15:30 | Part 4 Introduction to data interpretation & stats | Alyssa Imbert |
15:30 - 16:00 | Tea/coffee break | |
16:00 - 17:30 | Part 4 Introduction to data interpretation & stats | Alyssa Imbert |
18:30 | Evening meal |
Day 3 – Thursday 7th February 2019 |
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09:30 - 10:30 | Part 5 Metabolite Annotation | Steffen Neumann |
10:30 - 11:00 | Tea/coffee break | |
11:00 - 13:00 | Part 5 Metabolite Annotation | Steffen Neumann |
13:00 - 14:00 | Lunch | |
14:00 - 15:00 | Hands-on session | Steffen Neumann |
15:00 - 15:30 | Tea/coffee break | |
15:30 - 16:30 |
Introduction to MetaboLights database MetaboLights Labs - Workspace |
MetaboLights Team |
16:30 - 17:30 | Posters | |
18:30 | Evening meal |
Day 4 – Friday 8th February 2019 |
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09:30 - 10:30 | MetaboLights study submission | MetaboLights Team |
10:30 - 11:00 | Tea/coffee break | |
11:00 - 12:30 | Clinical case study | Kim Kultima |
12:30 - 13:30 | Lunch | |
13:30 - 15:00 | Clinical case study | Kim Kultima |
15:00 - 15:30 | Wrap-up and course feedback | Alexandra Holinski |
15:30 | Coach to Cambridge train station |
In order to be considered for a place on this course, applicants must do the following:
- Complete the online application form
- Submit a WORD document to johanna@ebi.ac.uk containing 200 words for your biography and work history and a 100 words on your current research interests (300 words total).
- Provide a letter of support from a supervisor or a senior co-worker, explaining why you should be selected for this course
Further details will be provided in the confirmation email after completing the online form
Incomplete applications will NOT be considered. Please read through the following guidance document for more information on how to complete your application.