Virtual course
Microscopy data analysis: machine learning and the BioImage Archive
2025
This virtual course will show how public bioimaging data resources, centred around the BioImage Archive, enable and enhance machine learning based image analysis. The content will explore a variety of data types including electron and light microscopy and miscellaneous or multi-modal imaging data at the cell and tissue scale. Participants will cover contemporary biological image analysis with an emphasis on machine learning methods, as well as how to access and use images from databases.
Virtual course
This course will be a virtual event delivered via a mixture of live-streamed sessions, pre-recorded lectures, and tutorials with live support. We will be using Zoom to run the live sessions (all fully password protected with automated English closed captioning and transcription) with support and both scientific and social networking opportunities provided by Slack and other methods, taking different time zones into account.
In order to make the most out of the course, you should make sure to have a stable internet connection throughout the week and are available between 08:00 – 18:00 BST each day. In the week before the course there will be a brief induction session. Computational practicals will run on EMBL-EBI's virtual training infrastructure, meaning participants will not require access to a powerful computer or install complex software on their own machines.
Selected participants may be sent materials prior to the course. These might include pre-recorded talks and required reading or online training that will be essential to fully engage with the course.
Who is this course for?
This course is aimed at scientists working with biomage data across the life sciences. It is suitable for those involved in creating bioimages or taking their first steps in analysis. The content is also suitable for those wanting to learn more about the BioImage Archive and gain experience with machine learning approaches for image analysis. The programme will be of particular interest to bioimage analysts with questions relating to the use of ‘big data’ and using the wealth of publically available data curated in the BioImage Archive.
The course is accessible to members of the bioimaging community and does not require prior experience with machine learning methods or use of the BioImage Archive. Applicants are encouraged to explore the resources below before starting their application. Applicants should be comfortable with basic programming tasks and have experience working with Python.
Recommended preparatory reading:
- BioImage Archive: A call for public archives for biological image data
- ZeroCostDL4Mic: an open platform to simplify access and use of Deep-Learning in Microscopy
- EMPIAR: a public archive for raw electron microscopy image data
- Image Data Resource: a bioimage data integration and publication platform
- BioImage Model Zoo
- OME-Zarr: a cloud-optimized bioimaging file format with international community support
What will I learn?
Learning outcomes
After the course you should be able to:
- Interact programmatically with the BioImage Archive and other data resources
- Interact with data formats for biological imaging data, particularly cloud-optimised formats
- Apply pre-built machine learning models to image data
- Train and retrain machine learning models on image data
- Utilise machine learning approaches for object detection, image segmentation, and de-noising
- Generate quantitative conclusions from images
Course content
During this course you will learn about:
Data repositories
Analysis tools
Analysis tools are subject to minor changes
Trainers
Programme
All times in the programme are listed in BST. Programme is subject to minor changes.
Time
Topic
Trainer
Day one – Monday 31 March 2025
Bioimage data resources
09:30 – 10:30
Arrival, welcome and introduction
Patricia Carvajal-López
10:30 – 11:00
General introduction
Matthew Hartley and Virginie Uhlmann
11:00 – 12:30
Bioimaging data formats - introduction, metadata and ontologies
EMBL-EBI Teams
12:30 — 13:30
Lunch break
13:30 — 14:30
BioImaging data resources at EMBL-EBI – an overview of the BioImage Archive (BIA) and EMPIAR
Mattew Hartley
14:30 — 15:00
Break
15:00 — 16:00
Image Data Resource (IDR) – introduction
16:00 — 16:30
Break
16:30 — 17:30 Flash talks 17:30
End of day
Day two – Tuesday 1 April 2025
Machine learning resources for bioimage analysis
09:00 — 09:10
Arrival and questions from previous sessions
09:10 – 10:10
Deep learning introduction
TBA
10:10 — 10:40
Break
10:40 – 11:20
Cellpose lecture
TBA
11:20 – 12:10 ilastik lecture TBA 12:00 — 13:00
Lunch break
13:00 — 14:00
CellProfiler lecture
TBA
14:00 — 14:30 Flash talks 14:30 — 15:00
Break
15:30 — 17:00 CellProfiler practical TBA 17:00 — 17:30
Group challenges introduction
Scientific organisers
17:30
End of day
Day three – Wednesday 2 April 2025
Machine learning resources for bioimage analysis
09:00 — 09:10
Arrival and questions from previous sessions
09:10 – 10:10
ZeroCost lecture TBA
10:10 — 10:40
Break
10:40 – 12:00
ZeroCost practical
TBA
12:00 — 13:00
Lunch break
13:00 – 14:00
Bioimage Model Zoo lecture
TBA
14:00 — 14:30
Break
14:30 — 16:00
Bioimage Model Zoo practical
TBA
16:00 — 16:30
Break
16:30 — 17:30 Group challenges work 17:30
End of day
Day four – Thursday 3 April 2025
Using machine learning resources for bioimage analysis on bioimage data resources
09:00 — 09:10
Arrival and questions from previous sessions
09:10 – 10:10
BioImaging data resources at EMBL-EBI – practicals
BIA Team
10:30 — 10:30
Break
10:35 – 12:00
Image data resource – practical
IDR Team
12:00 — 13:00
Lunch break
13:00 — 14:30
Interaction with imaging repositories - IDR+BIA
BIA and IDR Teams
14:30 — 15:00
Break
15:00 – 17:30
Group challenge work
17:30
End of day
Day five – Friday 4 April 2025
Advanced topics in bioimage analsys and group challenges
09:00 — 09:10
Arrival and questions from previous sessions
09:10 – 10:10
Advanced topics in bioimage analysis
TBA
10:10 — 10:40
Break
10:40 – 12:00
Group challenges work
12:00 — 13:00
Lunch break
13:00 — 14:00 Group challenges presentations 14:00 — 14:30
Break
14:30 — 15:30 Group challenges presentations 15:30 – 16:00
Final discussion and course wrap up
Patricia Carvajal-López 16:00
End of day
Please read our support page before starting your application. In order to be considered for a place on this course, you must do the following:
- Complete the online application form.
- Ensure you add relevant information to the ‘submission details’ section where you are asked to provide information on your:
- pre-requisite skills and knowledge
- current work and course expectations
- data availability
- Upload one letter of support from your supervisor or a senior colleague detailing reasons why you should be selected for the course.
Please submit all documents during the application process by 23:59 on 5 January 2025. Items marked * in the application are mandatory. Incomplete applications will not be processed.
All applicants will be informed of the status of their application (successful, waiting list, unsuccessful) by 20 January 2025. If you have any questions regarding the application process please contact Meredith Willmott.
Registration fees
The registration fee includes:
- access to the full five-day course programme
- access to a bespoke online course handbook
- secure community space on Slack
- virtual machine to work on that is accessed via the internet
- dedicated online support team
- course certificate on completion of the course
Academia £225.00 Industry £325.00
Financial assistance
Financial assistance is available for a limited number of participants on this course.
Registration fee waivers
We are able to offer a limited number of registration fee waivers for this course. If you receive a waiver, your registration fee will be reimbursed after you have completed the course.
You will need to apply for the fee waiver at the same time as submitting your application for the course, explaining why you require the waiver and how attending this course will benefit your career.
You will be informed about whether you have received the waiver at the same time as you hear about the application outcome for the course. If your course application is successful, you will need to pay the registration fee at the time you register. You will receive the waiver within a month of submitting your form.
Terms and conditions
The scientific organisers will select the recipients of financial assistance during the course application selection process. Selection for financial support is based on scientific merit, your current work study or location, the reasons for needing financial support and the impact this event will have on your career. Priority will be given to applications from low and middle income countries.
Application for financial support will not affect the outcome of your course application.
Course materials
The course materials from the 2023 edition of the course are now live and available for you to use. They provide a mixture of pre-recorded lectures, presentations, and practicals from the course, and will give you a snapshot of what to expect in the 2024 edition.
Flash talks
All participants will be asked to give a short presentation about their research work as part of the course. These provide an opportunity to share their research with the other participants and provide a forum for discussion. Successful applicants will be required to submit their talks upon registration.
AI4Life
Some of this course’s organisers and trainers are partners of the AI4Life Project. AI4Life has received funding from the European Union’s Horizon Europe research and innovation programme under grant agreement number 101057970.
Programme
All times in the programme are listed in BST. Programme is subject to minor changes.
Time | Topic | Trainer |
Day one – Monday 31 March 2025 Bioimage data resources | ||
09:30 – 10:30 | Arrival, welcome and introduction | Patricia Carvajal-López |
10:30 – 11:00 | General introduction | Matthew Hartley and Virginie Uhlmann |
11:00 – 12:30 | Bioimaging data formats - introduction, metadata and ontologies | EMBL-EBI Teams |
12:30 — 13:30 | Lunch break | |
13:30 — 14:30 | BioImaging data resources at EMBL-EBI – an overview of the BioImage Archive (BIA) and EMPIAR | Mattew Hartley |
14:30 — 15:00 | Break | |
15:00 — 16:00 | Image Data Resource (IDR) – introduction | |
16:00 — 16:30 | Break | |
16:30 — 17:30 | Flash talks | |
17:30 | End of day | |
Day two – Tuesday 1 April 2025 Machine learning resources for bioimage analysis | ||
09:00 — 09:10 | Arrival and questions from previous sessions | |
09:10 – 10:10 | Deep learning introduction | TBA |
10:10 — 10:40 | Break | |
10:40 – 11:20 | Cellpose lecture | TBA |
11:20 – 12:10 | ilastik lecture | TBA |
12:00 — 13:00 | Lunch break | |
13:00 — 14:00 | CellProfiler lecture | TBA |
14:00 — 14:30 | Flash talks | |
14:30 — 15:00 | Break | |
15:30 — 17:00 | CellProfiler practical | TBA |
17:00 — 17:30 | Group challenges introduction | Scientific organisers |
17:30 | End of day | |
Day three – Wednesday 2 April 2025 Machine learning resources for bioimage analysis | ||
09:00 — 09:10 | Arrival and questions from previous sessions | |
09:10 – 10:10 | ZeroCost lecture | TBA |
10:10 — 10:40 | Break | |
10:40 – 12:00 | ZeroCost practical | TBA |
12:00 — 13:00 | Lunch break | |
13:00 – 14:00 | Bioimage Model Zoo lecture | TBA |
14:00 — 14:30 | Break | |
14:30 — 16:00 | Bioimage Model Zoo practical | TBA |
16:00 — 16:30 | Break | |
16:30 — 17:30 | Group challenges work | |
17:30 | End of day | |
Day four – Thursday 3 April 2025 Using machine learning resources for bioimage analysis on bioimage data resources | ||
09:00 — 09:10 | Arrival and questions from previous sessions | |
09:10 – 10:10 | BioImaging data resources at EMBL-EBI – practicals | BIA Team |
10:30 — 10:30 | Break | |
10:35 – 12:00 | Image data resource – practical | IDR Team |
12:00 — 13:00 | Lunch break | |
13:00 — 14:30 | Interaction with imaging repositories - IDR+BIA | BIA and IDR Teams |
14:30 — 15:00 | Break | |
15:00 – 17:30 | Group challenge work |
|
17:30 | End of day | |
Day five – Friday 4 April 2025 Advanced topics in bioimage analsys and group challenges | ||
09:00 — 09:10 | Arrival and questions from previous sessions | |
09:10 – 10:10 | Advanced topics in bioimage analysis | TBA |
10:10 — 10:40 | Break | |
10:40 – 12:00 | Group challenges work |
|
12:00 — 13:00 | Lunch break | |
13:00 — 14:00 | Group challenges presentations | |
14:00 — 14:30 | Break | |
14:30 — 15:30 | Group challenges presentations | |
15:30 – 16:00 | Final discussion and course wrap up | Patricia Carvajal-López |
16:00 | End of day |
Please read our support page before starting your application. In order to be considered for a place on this course, you must do the following:
- Complete the online application form.
- Ensure you add relevant information to the ‘submission details’ section where you are asked to provide information on your:
- pre-requisite skills and knowledge
- current work and course expectations
- data availability
- Upload one letter of support from your supervisor or a senior colleague detailing reasons why you should be selected for the course.
Please submit all documents during the application process by 23:59 on 5 January 2025. Items marked * in the application are mandatory. Incomplete applications will not be processed.
All applicants will be informed of the status of their application (successful, waiting list, unsuccessful) by 20 January 2025. If you have any questions regarding the application process please contact Meredith Willmott.
Registration fees
The registration fee includes:
- access to the full five-day course programme
- access to a bespoke online course handbook
- secure community space on Slack
- virtual machine to work on that is accessed via the internet
- dedicated online support team
- course certificate on completion of the course
Academia | £225.00 |
Industry | £325.00 |
Financial assistance
Financial assistance is available for a limited number of participants on this course.
Registration fee waivers
We are able to offer a limited number of registration fee waivers for this course. If you receive a waiver, your registration fee will be reimbursed after you have completed the course.
You will need to apply for the fee waiver at the same time as submitting your application for the course, explaining why you require the waiver and how attending this course will benefit your career.
You will be informed about whether you have received the waiver at the same time as you hear about the application outcome for the course. If your course application is successful, you will need to pay the registration fee at the time you register. You will receive the waiver within a month of submitting your form.
Terms and conditions
The scientific organisers will select the recipients of financial assistance during the course application selection process. Selection for financial support is based on scientific merit, your current work study or location, the reasons for needing financial support and the impact this event will have on your career. Priority will be given to applications from low and middle income countries.
Application for financial support will not affect the outcome of your course application.
Course materials
The course materials from the 2023 edition of the course are now live and available for you to use. They provide a mixture of pre-recorded lectures, presentations, and practicals from the course, and will give you a snapshot of what to expect in the 2024 edition.
Flash talks
All participants will be asked to give a short presentation about their research work as part of the course. These provide an opportunity to share their research with the other participants and provide a forum for discussion. Successful applicants will be required to submit their talks upon registration.
AI4Life
Some of this course’s organisers and trainers are partners of the AI4Life Project. AI4Life has received funding from the European Union’s Horizon Europe research and innovation programme under grant agreement number 101057970.