- Course overview
- Search within this course
- Why Ensembl?
- What is Ensembl?
- When to use Ensembl
- How to access Ensembl
- How to search Ensembl
- Exploring sources of biological data
- Navigating Ensembl
- Customise Ensembl
- Manage your data
- Export data
- Download data with BioMart
- Bulk download genome-wide data files with FTP
- Ensembl tools
- Summary
- Exercises
- Your feedback
- Get help and support on Ensembl
- Acknowledgements
- Learn more
All materials are free cultural works licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, except where further licensing details are provided.
Finding cDNA sequence for a gene
Background
The human OSM (oncostatin M) gene is involved in regulation of cell growth. It regulates cytokine production, and is involved in the maturation of foetal hepatocytes. The OSM protein stimulates proliferation of Kaposi’s sarcoma cells in HIV-infected individuals.
Scenario
Imagine that you are working with the OSM gene, studying effects of mutation on the protein sequence. You would like to get the cDNA sequence, to make targetted mutations in the DNA that will code for alternative amino acids. You can use Ensembl to search for the OSM gene and find answers to these questions.