- Course overview
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- Introduction
- Real-time PCR
- Microarrays
- What is Next Generation DNA Sequencing?
- Biological interpretation of gene expression data
- Genotyping, epigenetic and DNA/RNA-protein interaction methods
- DNA/RNA-protein interactions
- Summary
- Quiz: Check your learning
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- References
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Data analysis
Once sequencing is complete, raw sequence data (FASTQ files) must undergo several analysis steps. The exact methods vary depending on the experiment but include:
- quality control
- read alignment (with or without a reference genome)
- quantification of gene and transcript expression
- and detection of differentially expressed genes (8,9)
We will go through each of these steps in more detail over the next few pages.