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Methods in proteomics
Several high-throughput technologies have been developed to investigate proteomes in depth. The most commonly applied are mass spectrometry (MS)-based techniques such as Tandem-MS and gel-based techniques such as differential in-gel electrophoresis (DIGE). These high-throughput technologies generate huge amounts of data. Databases are critical for recording and carefully storing this data, allowing the researcher to make connections between their results and existing knowledge.
Learn more about mass spectrometry with the recorded webinar Discovering biological information from mass spectrometry-based proteomics.
EMBL-EBI hosts up-to-date and accurate databases to enable rapid searching and retrieval of these data. The four major databases related to proteomic research (UniProtKB, IntAct, Reactome and PRIDE) are described in the next section. These four databases (especially UniProtKB) draw from gene sequence data (e.g. Ensembl) and annotation tools (e.g. InterPro) also hosted by EMBL-EBI.
Continue this online tutorial to learn more about these four data resources.