EMD-0836

Single-particle
3.63 Å
EMD-0836 Deposition: 22/10/2019
Map released: 15/04/2020
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0836

Structure of SMG1

EMD-0836

Single-particle
3.63 Å
EMD-0836 Deposition: 22/10/2019
Map released: 15/04/2020
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens
Sample: SMG1
Fitted models: 6l53 (Avg. Q-score: 0.343)

Deposition Authors: Xu Y , Qi Y
Cryo-EM structure of SMG1-SMG8-SMG9 complex.
Zhu L , Li L, Qi Y, Yu Z , Xu Y
(2019) Cell Res , 29 , 1027 - 1034
PUBMED: 31729466
DOI: doi:10.1038/s41422-019-0255-3
ISSN: 1001-0602
Abstract:
Nonsense-mediated mRNA decay (NMD) targets premature stop codon (PTC)-containing mRNAs for rapid degradation, and is essential for mammalian embryonic development, brain development and modulation of the stress response. The key event in NMD is the SMG1-mediated phosphorylation of an RNA helicase UPF1 and SMG1 kinase activity is inhibited by SMG8 and SMG9 in an unknown mechanism. Here, we determined the cryo-EM structures of human SMG1 at 3.6 Å resolution and the SMG1-SMG8-SMG9 complex at 3.4 Å resolution, respectively. SMG8 has a C-terminal kinase inhibitory domain (KID), which covers the catalytic pocket and inhibits the kinase activity of SMG1. Structural analyses suggest that GTP hydrolysis of SMG9 would lead to a dramatic conformational change of SMG8-SMG9 and the KID would move away from the inhibitory position to restore SMG1 kinase activity. Thus, our structural and biochemical analyses provide a mechanistic understanding of SMG1-SMG8-SMG9 complex assembly and the regulatory mechanism of SMG1 kinase activity.