EMD-10762

Single-particle
3.48 Å
EMD-10762 Deposition: 12/03/2020
Map released: 08/04/2020
Last modified: 23/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10762

Mammalian 48S late-stage translation initiation complex with histone 4 mRNA

EMD-10762

Single-particle
3.48 Å
EMD-10762 Deposition: 12/03/2020
Map released: 08/04/2020
Last modified: 23/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Oryctolagus cuniculus, Mus musculus
Sample: LS48S IC with histone 4 mRNA
Fitted models: 6yan (Avg. Q-score: 0.351)

Deposition Authors: Bochler A, Simonetti A
Structural Insights into the Mammalian Late-Stage Initiation Complexes.
Simonetti A , Guca E , Bochler A, Kuhn L , Hashem Y
(2020) Cell Rep , 31 , 107497 - 107497
PUBMED: 32268096
DOI: doi:10.1016/j.celrep.2020.03.061
ISSN: 2211-1247
Abstract:
In higher eukaryotes, the mRNA sequence in the direct vicinity of the start codon, called the Kozak sequence (CRCCaugG, where R is a purine), is known to influence the rate of the initiation process. However, the molecular basis underlying its role remains poorly understood. Here, we present the cryoelectron microscopy (cryo-EM) structures of mammalian late-stage 48S initiation complexes (LS48S ICs) in the presence of two different native mRNA sequences, β-globin and histone 4, at overall resolution of 3 and 3.5 Å, respectively. Our high-resolution structures unravel key interactions from the mRNA to eukaryotic initiation factors (eIFs): 1A, 2, 3, 18S rRNA, and several 40S ribosomal proteins. In addition, we are able to study the structural role of ABCE1 in the formation of native 48S ICs. Our results reveal a comprehensive map of ribosome/eIF-mRNA and ribosome/eIF-tRNA interactions and suggest the impact of mRNA sequence on the structure of the LS48S IC.