EMD-10836

Single-particle
3.3 Å
EMD-10836 Deposition: 07/04/2020
Map released: 29/07/2020
Last modified: 22/05/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10836

Rix1-Rea1 pre-60S particle - Rix1-subcomplex, body 3 (rigid body refinement)

EMD-10836

Single-particle
3.3 Å
EMD-10836 Deposition: 07/04/2020
Map released: 29/07/2020
Last modified: 22/05/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Saccharomyces cerevisiae
Sample: Rix1-Rea1 pre-60S assembly particle - Rix1-subcomplex
Fitted models: 6yle (Avg. Q-score: 0.496)

Deposition Authors: Kater L, Beckmann R
Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.
Kater L, Mitterer V , Thoms M, Cheng J, Berninghausen O, Beckmann R, Hurt E
(2020) Mol Cell , 79 , 615 - 628.e5
PUBMED: 32668200
DOI: doi:10.1016/j.molcel.2020.06.032
ISSN: 1097-2765
ASTM: MOCEFL
Abstract:
Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S intermediates were discovered that represent previously unknown states after Rea1-mediated removal of the Ytm1-Erb1 complex and reveal how the L1 stalk develops from a pre-mature nucleolar to a mature-like nucleoplasmic state. A later pre-60S intermediate shows how the central protuberance arises, assisted by the nearby Rix1-Rea1 machinery, which was solved in its pre-ribosomal context to molecular resolution. This revealed a Rix12-Ipi32 tetramer anchored to the pre-60S via Ipi1, strategically positioned to monitor this decisive remodeling. These results are consistent with a general underlying principle that temporarily stabilized immature RNA domains are successively remodeled by assembly factors, thereby ensuring failsafe assembly progression.