EMD-11375

Subtomogram averaging
28.0 Å
EMD-11375 Deposition: 10/07/2020
Map released: 21/07/2021
Last modified: 09/02/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11375

In cellulo nuclear pore complex in constricted conformation from energy depleted S. pombe cells

EMD-11375

Subtomogram averaging
28.0 Å
EMD-11375 Deposition: 10/07/2020
Map released: 21/07/2021
Last modified: 09/02/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Schizosaccharomyces pombe
Sample: energy depleted S. pombe cells

Deposition Authors: Zimmerli CE , Allegretti M , Rantos V , Goetz S, Obarska-Kosinska A, Zagoriy I , Hummer G , Mahamid J , Kosinski J , Beck M
Nuclear pores dilate and constrict in cellulo.
PUBMED: 34762489
DOI: doi:10.1126/science.abd9776
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
In eukaryotic cells, nuclear pore complexes (NPCs) fuse the inner and outer nuclear membranes and mediate nucleocytoplasmic exchange. They are made of 30 different nucleoporins and form a cylindrical architecture around an aqueous central channel. This architecture is highly dynamic in space and time. Variations in NPC diameter have been reported, but the physiological circumstances and the molecular details remain unknown. Here, we combined cryo–electron tomography with integrative structural modeling to capture a molecular movie of the respective large-scale conformational changes in cellulo. Although NPCs of exponentially growing cells adopted a dilated conformation, they reversibly constricted upon cellular energy depletion or conditions of hypertonic osmotic stress. Our data point to a model where the nuclear envelope membrane tension is linked to the conformation of the NPC.