EMD-11842

Single-particle
3.9 Å
EMD-11842 Deposition: 14/10/2020
Map released: 24/02/2021
Last modified: 09/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11842

Schizosaccharomyces pombe RNA polymerase I (elongation complex)

EMD-11842

Single-particle
3.9 Å
EMD-11842 Deposition: 14/10/2020
Map released: 24/02/2021
Last modified: 09/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843), synthetic construct
Sample: S. pombe RNA polymerase I - elongation complex
Fitted models: 7aoe (Avg. Q-score: 0.422)

Deposition Authors: Heiss F, Daiss J
Conserved strategies of RNA polymerase I hibernation and activation.
Heiss FB , Daiss JL, Becker P, Engel C
(2021) Nat Commun , 12 , 758 - 758
PUBMED: 33536435
DOI: doi:10.1038/s41467-021-21031-8
ISSN: 2041-1723
Abstract:
RNA polymerase (Pol) I transcribes the ribosomal RNA precursor in all eukaryotes. The mechanisms 'activation by cleft contraction' and 'hibernation by dimerization' are unique to the regulation of this enzyme, but structure-function analysis is limited to baker's yeast. To understand whether regulation by such strategies is specific to this model organism or conserved among species, we solve three cryo-EM structures of Pol I from Schizosaccharomyces pombe in different functional states. Comparative analysis of structural models derived from high-resolution reconstructions shows that activation is accomplished by a conserved contraction of the active center cleft. In contrast to current beliefs, we find that dimerization of the S. pombe polymerase is also possible. This dimerization is achieved independent of the 'connector' domain but relies on two previously undescribed interfaces. Our analyses highlight the divergent nature of Pol I transcription systems from their counterparts and suggest conservation of regulatory mechanisms among organisms.