EMD-12818

Single-particle
2.8 Å
EMD-12818 Deposition: 28/04/2021
Map released: 26/01/2022
Last modified: 20/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-12818

SARS CoV-2 Spike protein, Bristol UK Deletion variant, Closed conformation, C3 symmetry

EMD-12818

Single-particle
2.8 Å
EMD-12818 Deposition: 28/04/2021
Map released: 26/01/2022
Last modified: 20/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Severe acute respiratory syndrome coronavirus 2
Sample: SARS CoV-2 Spike protein, Bristol UK Deletion variant, Closed conformation, C3 symmetry
Fitted models: 7od3 (Avg. Q-score: 0.622)

Deposition Authors: Toelzer C , Gupta K
Structural insights in cell-type specific evolution of intra-host diversity by SARS-CoV-2.
PUBMED: 35017512
DOI: doi:10.1038/s41467-021-27881-6
ISSN: 2041-1723
Abstract:
As the global burden of SARS-CoV-2 infections escalates, so does the evolution of viral variants with increased transmissibility and pathology. In addition to this entrenched diversity, RNA viruses can also display genetic diversity within single infected hosts with co-existing viral variants evolving differently in distinct cell types. The BriSΔ variant, originally identified as a viral subpopulation from SARS-CoV-2 isolate hCoV-19/England/02/2020, comprises in the spike an eight amino-acid deletion encompassing a furin recognition motif and S1/S2 cleavage site. We elucidate the structure, function and molecular dynamics of this spike providing mechanistic insight into how the deletion correlates to viral cell tropism, ACE2 receptor binding and infectivity of this SARS-CoV-2 variant. Our results reveal long-range allosteric communication between functional domains that differ in the wild-type and the deletion variant and support a view of SARS-CoV-2 probing multiple evolutionary trajectories in distinct cell types within the same infected host.