EMD-12992
SARS-CoV-2 spike subtomogram average from intracelullar viruses
EMD-12992
Subtomogram averaging16.0 Å
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Map released: 23/06/2021
Last modified: 13/10/2021
Sample Organism:
Severe acute respiratory syndrome coronavirus 2
Sample: Severe acute respiratory syndrome coronavirus 2
Raw data: EMPIAR-10753
Deposition Authors: Mendonca L, Zhang P
Sample: Severe acute respiratory syndrome coronavirus 2
Raw data: EMPIAR-10753
Deposition Authors: Mendonca L, Zhang P
Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress.
Mendonca L
,
Howe A
,
Gilchrist JB
,
Sheng Y
,
Sun D
,
Knight ML
,
Zanetti-Domingues LC
,
Bateman B
,
Krebs AS
,
Chen L
,
Radecke J
,
Li VD
,
Ni T
,
Kounatidis I,
Koronfel MA
,
Szynkiewicz M,
Harkiolaki M
,
Martin-Fernandez ML
,
James W
,
Zhang P
(2021) Nat Commun , 12 , 4629 - 4629
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(2021) Nat Commun , 12 , 4629 - 4629
Abstract:
Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events - e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules.
Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events - e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules.