EMD-12992

Subtomogram averaging
16.0 Å
EMD-12992 Deposition: 24/05/2021
Map released: 23/06/2021
Last modified: 13/10/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-12992

SARS-CoV-2 spike subtomogram average from intracelullar viruses

EMD-12992

Subtomogram averaging
16.0 Å
EMD-12992 Deposition: 24/05/2021
Map released: 23/06/2021
Last modified: 13/10/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Severe acute respiratory syndrome coronavirus 2
Sample: Severe acute respiratory syndrome coronavirus 2
Raw data: EMPIAR-10753

Deposition Authors: Mendonca L, Zhang P
Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress.
PUBMED: 34330917
DOI: doi:10.1038/s41467-021-24887-y
ISSN: 2041-1723
Abstract:
Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events - e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules.