EMD-13088

Subtomogram averaging
7.0 Å
EMD-13088 Deposition: 15/06/2021
Map released: 18/08/2021
Last modified: 13/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-13088

Mature HIV-1 matrix structure

EMD-13088

Subtomogram averaging
7.0 Å
EMD-13088 Deposition: 15/06/2021
Map released: 18/08/2021
Last modified: 13/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Human immunodeficiency virus 1
Sample: Human immunodeficiency virus 1
Fitted models: 7ovr (Avg. Q-score: 0.245)

Deposition Authors: Qu K , Ke ZL, Zila V , Anders-Oesswein M, Glass B , Muecksch F, Mueller R, Schultz C , Mueller B, Kraeusslich HG, Briggs JAG
Maturation of the matrix and viral membrane of HIV-1.
Qu K , Ke Z , Zila V , Anders-Osswein M, Glass B , Mucksch F , Muller R , Schultz C , Muller B , Krausslich HG , Briggs JAG
(2021) Science , 373 , 700 - 704
PUBMED: 34353956
DOI: doi:10.1126/science.abe6821
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
Gag, the primary structural protein of HIV-1, is recruited to the plasma membrane for virus assembly by its matrix (MA) domain. Gag is subsequently cleaved into its component domains, causing structural maturation to repurpose the virion for cell entry. We determined the structure and arrangement of MA within immature and mature HIV-1 through cryo-electron tomography. We found that MA rearranges between two different hexameric lattices upon maturation. In mature HIV-1, a lipid extends out of the membrane to bind with a pocket in MA. Our data suggest that proteolytic maturation of HIV-1 not only assembles the viral capsid surrounding the genome but also repurposes the membrane-bound MA lattice for an entry or postentry function and results in the partial removal of up to 2500 lipids from the viral membrane.