EMD-13473

Single-particle
3.2 Å
EMD-13473 Deposition: 25/08/2021
Map released: 27/04/2022
Last modified: 17/07/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-13473

Structure of the human CCAN deltaCT complex

EMD-13473

Single-particle
3.2 Å
EMD-13473 Deposition: 25/08/2021
Map released: 27/04/2022
Last modified: 17/07/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens
Sample: Human inner kinetochore CCAN
Fitted models: 7pkn (Avg. Q-score: 0.319)

Deposition Authors: Muir KW , Yatskevich S , Bellini D , Barford D
Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome.
Yatskevich S , Muir KW , Bellini D , Zhang Z , Yang J , Tischer T , Predin M , Dendooven T , McLaughlin SH , Barford D
(2022) Science , 376 , 844 - 852
PUBMED: 35420891
DOI: doi:10.1126/science.abn3810
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-ANuc) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron microscopy structures of the human inner kinetochore constitutive centromere associated network (CCAN) complex bound to CENP-ANuc reconstituted onto α-satellite DNA. CCAN forms edge-on contacts with CENP-ANuc, and a linker DNA segment of the α-satellite repeat emerges from the fully wrapped end of the nucleosome to thread through the central CENP-LN channel that tightly grips the DNA. The CENP-TWSX histone-fold module further augments DNA binding and partially wraps the linker DNA in a manner reminiscent of canonical nucleosomes. Our study suggests that the topological entrapment of the linker DNA by CCAN provides a robust mechanism by which kinetochores withstand both pushing and pulling forces exerted by the mitotic spindle.